Pseudomonas aeruginosa PAO1, PA2648 (nuoM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2648
Name
nuoM
Replicon chromosome
Genomic location 2994728 - 2996257 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251338.1
GI 15597844
Affymetrix PA2648_nuoM_at
Entrez 882357
GenBank AAG06036.1
INSDC AAG06036.1
NCBI Locus Tag PA2648
protein_id(GenBank) gb|AAG06036.1|AE004693_7|gnl|PseudoCAP|PA2648
TIGR NTL03PA02648
UniParc UPI00000C5788
UniProtKB Acc Q9I0J0
UniProtKB ID NUOM_PSEAE
UniRef100 UniRef100_Q9I0J0
UniRef50 UniRef50_P0AFE9
UniRef90 UniRef90_Q9I0J0

Product

Feature Type CDS
Coding Frame 1
Product Name
NADH dehydrogenase I chain M
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.78
Kyte-Doolittle Hydrophobicity Value 0.948
Molecular Weight (kDa) 55.7
Isoelectric Point (pI) 7.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 557 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002907 (517 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2648
Search term: nuoM

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 4 [Source:HGNC Symbol;Acc:HGNC:7459]
E-value: 2.5e-38
Percent Identity: 31.8

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Escherichia coli mutants lacking NADH dehydrogenase I have a competitive disadvantage in stationary phase.
Zambrano MM, Kolter R
J Bacteriol 1993 Sep;175(17):5642-7
PubMed ID: 8366049
The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.
Weidner U, Geier S, Ptock A, Friedrich T, Leif H, Weiss H
J Mol Biol 1993 Sep 5;233(1):109-22
PubMed ID: 7690854