Pseudomonas aeruginosa PAO1, PA2688 (pfeA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2688
Name
pfeA
Replicon chromosome
Genomic location 3040242 - 3042482 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 13 transposon mutants in orthologs

Cross-References

RefSeq NP_251378.1
GI 15597884
Affymetrix PA2688_pfeA_at
Entrez 882784
GenBank AAG06076.1
NCBI Locus Tag PA2688
protein_id(GenBank) gb|AAG06076.1|AE004697_1|gnl|PseudoCAP|PA2688
TIGR NTL03PA02688
UniProtKB Acc Q05098
UniProtKB ID PFEA_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
Ferric enterobactin receptor, outer membrane protein PfeA precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -9.70
Kyte-Doolittle Hydrophobicity Value -0.557
Molecular Weight (kDa) 81.0
Isoelectric Point (pI) 5.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5MZS MEMBRANE PROTEIN 02/01/17 Crystal structure of the ferric enterobactin receptor (PfeA) mutant (R480A_Q482A) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.67 X-RAY DIFFRACTION 99.7
5NC3 MEMBRANE PROTEIN 03/03/17 Crystal structure of the ferric enterobactin receptor (PfeA) from Pseudomonas aeruginosa in complex with the tris-catechol vector Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.57 X-RAY DIFFRACTION 100.0
7OBW MEMBRANE PROTEIN 04/23/21 Crystal structure of the ferric enterobactin receptor (PfeA) from Pseudomonas aeruginosa in complex with TCV-L6 Pseudomonas aeruginosa PAO1 2.66 X-RAY DIFFRACTION 100.0
5NR2 MEMBRANE PROTEIN 04/21/17 Crystal structure of the ferric enterobactin receptor (PfeA) from Pseudomonas aeruginosa in complex with azotochelin Pseudomonas aeruginosa 2.78 X-RAY DIFFRACTION 100.0
5OUT MEMBRANE PROTEIN 08/24/17 CRYSTAL STRUCTURE OF THE FERRIC ENTEROBACTIN RECEPTOR (PFEA) MUTANT (G324V) FROM PSEUDOMONAS AERUGINOSA Pseudomonas aeruginosa PAO1 2.9 X-RAY DIFFRACTION 99.9
6Z2N MEMBRANE PROTEIN 05/18/20 Crystal structure of the ferric enterobactin receptor (PfeA) in complex with BCV-L6 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.029 X-RAY DIFFRACTION 100.0
6R1F MEMBRANE PROTEIN 03/14/19 Crystal structure of the ferric enterobactin receptor mutant R480A from Pseudomonas aeruginosa (PfeA) in complex with enterobactin Pseudomonas aeruginosa PAO1 3.11 X-RAY DIFFRACTION 99.9
6I2J MEMBRANE PROTEIN 11/01/18 Crystal structure of the ferric enterobactin receptor mutant (Q482A) from Pseudomonas aeruginosa (PfeA) in complex with enterobactin Pseudomonas aeruginosa PAO1 2.96 X-RAY DIFFRACTION 99.9
6Z33 MEMBRANE PROTEIN 05/19/20 Crystal structure of the ferric enterobactin receptor (PfeA) in complex with BCV Pseudomonas aeruginosa PAO1 2.711 X-RAY DIFFRACTION 100.0
5NC4 MEMBRANE PROTEIN 03/03/17 Crystal structure of the ferric enterobactin receptor (PfeA) in complex with protochelin from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.8 X-RAY DIFFRACTION 100.0
5M9B MEMBRANE PROTEIN 11/01/16 Crystal structure of the ferric enterobactin receptor (PfeA) from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.12 X-RAY DIFFRACTION 100.0
6YY5 MEMBRANE PROTEIN 05/04/20 Crystal structure of the ferric enterobactin receptor (PfeA) in complex with TCV_L5 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.717 X-RAY DIFFRACTION 100.0
6Q5E MEMBRANE PROTEIN 12/07/18 Crystal structure of the ferric enterobactin receptor from Pseudomonas aeruginosa (PfeA) in complex with enterobactin Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.7 X-RAY DIFFRACTION 100.0
6Y47 MEMBRANE PROTEIN 02/19/20 Crystal structure of the ferric enterobactin receptor (PfeA) in complex with BCV-L5 Pseudomonas aeruginosa PAO1 3.04 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 330 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000897 (3061 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2688
Search term: pfeA

Human Homologs

References

Enterobactin-mediated iron transport in Pseudomonas aeruginosa.
Poole K, Young L, Neshat S
J Bacteriol 1990 Dec;172(12):6991-6
PubMed ID: 2174865
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295