Pseudomonas aeruginosa PAO1, PA2689 (pfeE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2689
Name
pfeE
Replicon chromosome
Genomic location 3042502 - 3043416 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_251379.1
GI 15597885
Affymetrix PA2689_at
Entrez 882785
GenBank AAG06077.1
INSDC AAG06077.1
NCBI Locus Tag PA2689
protein_id(GenBank) gb|AAG06077.1|AE004697_2|gnl|PseudoCAP|PA2689
TIGR NTL03PA02689
UniParc UPI00000C57AE
UniProtKB Acc Q9I0F2
UniProtKB ID Q9I0F2_PSEAE
UniRef100 UniRef100_Q9I0F2
UniRef50 UniRef50_J3IWY7
UniRef90 UniRef90_Q9I0F2

Product

Feature Type CDS
Coding Frame 1
Product Name
esterase, PfeE
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) 2.96
Kyte-Doolittle Hydrophobicity Value -0.181
Molecular Weight (kDa) 32.7
Isoelectric Point (pI) 9.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
fusion protein localization evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6GI2 HYDROLASE 05/09/18 Crystal structure of the ferric enterobactin esterase (pfeE) mutant(S157A) from Pseudomonas aeruginosa in complex with Tris-catechol vector Pseudomonas aeruginosa 1.49 X-RAY DIFFRACTION 99.6
6GI5 HYDROLASE 05/09/18 Crystal structure of the ferric enterobactin esterase (PfeE) from Pseudomonas aeruginosa in complex with the tris-catechol vector Pseudomonas aeruginosa 3.11 X-RAY DIFFRACTION 100.0
6GI0 HYDROLASE 05/09/18 Crystal structure of the ferric enterobactin esterase (PfeE) from Pseudomonas aeruginosa Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0
6GI1 HYDROLASE 05/09/18 Crystal structure of the ferric enterobactin esterase (pfeE) mutant(S157A) from Pseudomonas aeruginosa in presence of enterobactin Pseudomonas aeruginosa PAO1 1.66 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 102 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002873 (383 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2689
Search term: pfeE
Search term: esterase, PfeE

Human Homologs

References

A Key Role for the Periplasmic PfeE Esterase in Iron Acquisition via the Siderophore Enterobactin in Pseudomonas aeruginosa.
Perraud Q, Moynié L, Gasser V, Munier M, Godet J, Hoegy F, Mély Y, Mislin GLA, Naismith JH, Schalk IJ
ACS Chem Biol 2018 Sep 21;13(9):2603-2614
PubMed ID: 30086222