Pseudomonas aeruginosa PAO1, PA2702 (tse2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2702
Name
tse2
Replicon chromosome
Genomic location 3056890 - 3057366 (+ strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251392.1
GI 15597898
Affymetrix PA2702_at
DNASU PaCD00007166
Entrez 880323
GenBank AAG06090.1
INSDC AAG06090.1
NCBI Locus Tag PA2702
protein_id(GenBank) gb|AAG06090.1|AE004698_6|gnl|PseudoCAP|PA2702
TIGR NTL03PA02702
UniParc UPI00000C57BA
UniProtKB Acc Q9I0E0
UniProtKB ID Q9I0E0_PSEAE
UniRef100 UniRef100_Q9I0E0
UniRef50 UniRef50_Q9I0E0
UniRef90 UniRef90_Q9I0E0

Product

Feature Type CDS
Coding Frame 1
Product Name
Tse2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.29
Kyte-Doolittle Hydrophobicity Value -0.556
Molecular Weight (kDa) 17.7
Isoelectric Point (pI) 6.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5AKO UNKNOWN FUNCTION 03/04/15 The complex of Tse2 and Tsi2 from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category Type VI secretion system translocated effectors
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Escherichia coli BL21
Notes E. coli BL21 transformed with tse2 had strongly inhibited growth. This was not observed when transformed with tse2 and tsi2 together.
Reference 20114026
Source 2
Database PseudoCAP
Category Type VI secretion system translocated effectors
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Burkholderia thailandensis E264
Notes Burkholderia thailandensis E264 transformed with tse2 had strongly inhibited growth. This was not observed when transformed with tse2 and tsi2 together.
Reference 20114026

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 2 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002862 (298 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2702
Search term: tse2
Search term: Tse2

Human Homologs

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026