Pseudomonas aeruginosa PAO1, PA2739 (pheT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2739
Name
pheT
Replicon chromosome
Genomic location 3100116 - 3102494 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251429.1
GI 15597935
Affymetrix PA2739_pheT_at
Entrez 882969
GenBank AAG06127.1
INSDC AAG06127.1
NCBI Locus Tag PA2739
protein_id(GenBank) gb|AAG06127.1|AE004702_4|gnl|PseudoCAP|PA2739
TIGR NTL03PA02739
UniParc UPI00000C57DE
UniProtKB Acc Q9I0A4
UniProtKB ID SYFB_PSEAE
UniRef100 UniRef100_Q9I0A4
UniRef50 UniRef50_P07395
UniRef90 UniRef90_Q9I0A4

Product

Feature Type CDS
Coding Frame 1
Product Name
phenylalanyl-tRNA synthetase, beta subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -21.78
Kyte-Doolittle Hydrophobicity Value -0.067
Molecular Weight (kDa) 86.8
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4P72 LIGASE/LIGASE INHIBITOR 03/25/14 PheRS in complex with compound 2a Pseudomonas aeruginosa 2.62 X-RAY DIFFRACTION 100.0
4P71 LIGASE 03/25/14 Apo PheRS from P. aeuriginosa Pseudomonas aeruginosa 2.79 X-RAY DIFFRACTION 100.0
4P73 LIGASE/LIGASE Inhibitor 03/25/14 PheRS in complex with compound 1a Pseudomonas aeruginosa 3.03 X-RAY DIFFRACTION 100.0
4P75 LIGASE/LIGASE INHIBITOR 03/25/14 PheRS in complex with compound 4a Pseudomonas aeruginosa 2.96 X-RAY DIFFRACTION 100.0
4P74 LIGASE/LIGASE INHIBITOR 03/25/14 PheRS in complex with compound 3a Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 660 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002832 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2739
Search term: pheT

Human Homologs

References

Sequence of the Escherichia coli pheST operon and identification of the himA gene.
Mechulam Y, Fayat G, Blanquet S
J Bacteriol 1985 Aug;163(2):787-91
PubMed ID: 2991205
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994