Pseudomonas aeruginosa PAO1, PA2740 (pheS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2740
Name
pheS
Replicon chromosome
Genomic location 3102529 - 3103545 (- strand)

Cross-References

RefSeq NP_251430.1
GI 15597936
Affymetrix PA2740_pheS_at
DNASU PaCD00007185
Entrez 882970
GenBank AAG06128.1
INSDC AAG06128.1
NCBI Locus Tag PA2740
protein_id(GenBank) gb|AAG06128.1|AE004702_5|gnl|PseudoCAP|PA2740
TIGR NTL03PA02740
UniParc UPI0000136422
UniProtKB Acc Q9I0A3
UniProtKB ID SYFA_PSEAE
UniRef100 UniRef100_Q9I0A3
UniRef50 UniRef50_Q5F9U0
UniRef90 UniRef90_Q9I0A3

Product

Feature Type CDS
Coding Frame 1
Product Name
phenylalanyl-tRNA synthetase, alpha-subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.58
Kyte-Doolittle Hydrophobicity Value -0.241
Molecular Weight (kDa) 38.1
Isoelectric Point (pI) 5.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4P75 LIGASE/LIGASE INHIBITOR 03/25/14 PheRS in complex with compound 4a Pseudomonas aeruginosa 2.96 X-RAY DIFFRACTION 100.0
4P74 LIGASE/LIGASE INHIBITOR 03/25/14 PheRS in complex with compound 3a Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 100.0
4P71 LIGASE 03/25/14 Apo PheRS from P. aeuriginosa Pseudomonas aeruginosa 2.79 X-RAY DIFFRACTION 100.0
4P73 LIGASE/LIGASE Inhibitor 03/25/14 PheRS in complex with compound 1a Pseudomonas aeruginosa 3.03 X-RAY DIFFRACTION 100.0
4P72 LIGASE/LIGASE INHIBITOR 03/25/14 PheRS in complex with compound 2a Pseudomonas aeruginosa 2.62 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 666 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002831 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2740
Search term: pheS

Human Homologs

References

Amino acid substrate specificity of Escherichia coli phenylalanyl-tRNA synthetase altered by distinct mutations.
Kast P, Hennecke H
J Mol Biol 1991 Nov 5;222(1):99-124
PubMed ID: 1942071
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994