Pseudomonas aeruginosa PAO1, PA2744 (thrS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2744
Name
thrS
Replicon chromosome
Genomic location 3104830 - 3106752 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_251434.1
GI 15597940
Affymetrix PA2744_thrS_at
Entrez 882972
GenBank AAG06132.1
INSDC AAG06132.1
NCBI Locus Tag PA2744
protein_id(GenBank) gb|AAG06132.1|AE004702_9|gnl|PseudoCAP|PA2744
TIGR NTL03PA02744
UniParc UPI00001366C8
UniProtKB Acc Q9I099
UniProtKB ID SYT_PSEAE
UniRef100 UniRef100_B7V188
UniRef50 UniRef50_Q83C10
UniRef90 UniRef90_A4XTS1

Product

Feature Type CDS
Coding Frame 1
Product Name
threonyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.90
Kyte-Doolittle Hydrophobicity Value -0.443
Molecular Weight (kDa) 73.1
Isoelectric Point (pI) 6.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002827 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2744
Search term: thrS

Human Homologs

Ensembl 110, assembly GRCh38.p14
threonyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:11572]
E-value: 6.9e-131
Percent Identity: 38.7
Ensembl 110, assembly GRCh38.p14
threonyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:11572]
E-value: 6.9e-131
Percent Identity: 38.7

References

Genetic definition of the translational operator of the threonine-tRNA ligase gene in Escherichia coli.
Springer M, Graffe M, Butler JS, Grunberg-Manago M
Proc Natl Acad Sci U S A 1986 Jun;83(12):4384-8
PubMed ID: 3086882
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994