Pseudomonas aeruginosa PAO1, PA2876 (pyrF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2876
Name
pyrF
Replicon chromosome
Genomic location 3229484 - 3230182 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251566.1
GI 15598072
Affymetrix PA2876_pyrF_at
DNASU PaCD00007256
Entrez 882585
GenBank AAG06264.1
INSDC AAG06264.1
NCBI Locus Tag PA2876
protein_id(GenBank) gb|AAG06264.1|AE004714_1|gnl|PseudoCAP|PA2876
TIGR NTL03PA02876
UniParc UPI0000132D29
UniProtKB Acc Q59654
UniProtKB ID PYRF_PSEAE
UniRef100 UniRef100_B7V800
UniRef50 UniRef50_P07691
UniRef90 UniRef90_Q884R0

Product

Feature Type CDS
Coding Frame 1
Product Name
orotidine 5'-phosphate decarboxylase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.46
Kyte-Doolittle Hydrophobicity Value 0.234
Molecular Weight (kDa) 24.4
Isoelectric Point (pI) 5.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4267
Category Uracil
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 393 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002695 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2876
Search term: pyrF

Human Homologs

References

Nucleotide sequence and characterization of the pyrF operon of Escherichia coli K12.
Turnbough CL Jr, Kerr KH, Funderburg WR, Donahue JP, Powell FE
J Biol Chem 1987 Jul 25;262(21):10239-45
PubMed ID: 2956254
Orotidine-5'-monophosphate decarboxylase from Pseudomonas aeruginosa PAO1: cloning, overexpression, and enzyme characterization.
Strych U, Wohlfarth S, Winkler UK
Curr Microbiol 1994 Dec;29(6):353-9
PubMed ID: 7765522