Pseudomonas aeruginosa PAO1, PA2939

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2939
Name
Synonym: pepB
Replicon chromosome
Genomic location 3294282 - 3295892 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_251629.1
GI 15598135
Affymetrix PA2939_at
Entrez 882792
GenBank AAG06327.1
INSDC AAG06327.1
NCBI Locus Tag PA2939
protein_id(GenBank) gb|AAG06327.1|AE004720_3|gnl|PseudoCAP|PA2939
TIGR NTL03PA02939
UniParc UPI00000C5896
UniProtKB Acc Q9HZQ8
UniProtKB ID LAP_PSEAE
UniRef100 UniRef100_Q02PA2
UniRef50 UniRef50_Q02PA2
UniRef90 UniRef90_Q02PA2

Product

Feature Type CDS
Coding Frame 1
Product Name
probable aminopeptidase
Product Name Confidence: Class 3
Synonyms pepB
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.72
Kyte-Doolittle Hydrophobicity Value -0.271
Molecular Weight (kDa) 57.5
Isoelectric Point (pI) 4.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8AC9 HYDROLASE 07/05/22 Structure of Pseudomonas aeruginosa aminopeptidase, PaAP_T Pseudomonas aeruginosa 2.351 X-RAY DIFFRACTION 99.4
8ACK HYDROLASE 07/05/22 Structure of Pseudomonas aeruginosa aminopeptidase, PaAP Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 1.784 X-RAY DIFFRACTION 99.4
8ACR HYDROLASE 07/06/22 Structure of Pseudomonas aeruginosa aminopeptidase, PaAP Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 98.8
8AC7 HYDROLASE 07/05/22 Structure of Pseudomonas aeruginosa aminopeptidase, PaAP Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0
8ACG HYDROLASE 07/05/22 Structure of Pseudomonas aeruginosa aminopeptidase, PaAP_T E340A mutant Pseudomonas aeruginosa 2.84 X-RAY DIFFRACTION 99.2

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 190 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002641 (321 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2939

Human Homologs

References

A secreted aminopeptidase of Pseudomonas aeruginosa. Identification, primary structure, and relationship to other aminopeptidases.
Cahan R, Axelrad I, Safrin M, Ohman DE, Kessler E
J Biol Chem 2001 Nov 23;276(47):43645-52
PubMed ID: 11533066