Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA2965
|
Name |
fabF1
|
Replicon | chromosome |
Genomic location | 3323574 - 3324818 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_251655.1 |
GI | 15598161 |
Affymetrix | PA2965_fabF1_at |
Entrez | 879894 |
GenBank | AAG06353.1 |
INSDC | AAG06353.1 |
NCBI Locus Tag | PA2965 |
protein_id(GenBank) | gb|AAG06353.1|AE004722_9|gnl|PseudoCAP|PA2965 |
TIGR | NTL03PA02965 |
UniParc | UPI00000D428F |
UniProtKB Acc | G3XDA2 |
UniProtKB ID | G3XDA2_PSEAE |
UniRef100 | UniRef100_G3XDA2 |
UniRef50 | UniRef50_Q9KQH9 |
UniRef90 | UniRef90_G3XDA2 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
beta-ketoacyl-acyl carrier protein synthase II
|
Synonyms |
FabF protein |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -6.74 |
Kyte-Doolittle Hydrophobicity Value | -0.029 |
Molecular Weight (kDa) | 43.5 |
Isoelectric Point (pI) | 5.81 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7OC0 | ANTIBIOTIC | 04/25/21 | Structure of Pseudomonas aeruginosa FabF mutant C164Q in complex with a ligand (2S,4R)-2-(thiophen-2-yl)thiazolidine-4-carboxylic acid | Pseudomonas aeruginosa PAO1 | 1.78 | X-RAY DIFFRACTION | 99.8 |
4B7V | TRANSFERASE | 08/22/12 | Structure of wild type Pseudomonas aeruginosa FabF (KASII) | PSEUDOMONAS AERUGINOSA | 1.73 | X-RAY DIFFRACTION | 100.0 |
4JB6 | TRANSFERASE | 02/19/13 | Structure of Pseudomonas aeruginosa FabF mutant C164Q | Pseudomonas aeruginosa | 2.4 | X-RAY DIFFRACTION | 99.8 |
4JPF | TRANSFERASE | 03/19/13 | Structure of wild type Pseudomonas aeruginosa FabF (KASII) in Complex with ligand | Pseudomonas aeruginosa | 1.67 | X-RAY DIFFRACTION | 100.0 |
7OC1 | ANTIBIOTIC | 04/25/21 | Structure of Pseudomonas aeruginosa FabF mutant C164Q in complex with Platensimycin | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 99.8 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 572 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002617 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA2965
Search term: fabF1
Search term: beta-ketoacyl-acyl carrier protein synthase II
|
Ensembl
110, assembly
GRCh38.p14
|
3-oxoacyl-ACP synthase, mitochondrial [Source:HGNC Symbol;Acc:HGNC:26063]
E-value:
3.2e-97
Percent Identity:
47.4
|
Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis.
Garwin JL, Klages AL, Cronan JE Jr
J Biol Chem 1980 Apr 25;255(8):3263-5
PubMed ID: 6988423
|
Cloning of the fabF gene in an expression vector and in vitro characterization of recombinant fabF and fabB encoded enzymes from Escherichia coli.
Edwards P, Nelsen JS, Metz JG, Dehesh K
FEBS Lett 1997 Jan 27;402(1):62-6
PubMed ID: 9013860
|
Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes.
Huang W, Jia J, Edwards P, Dehesh K, Schneider G, Lindqvist Y
EMBO J 1998 Mar 2;17(5):1183-91
PubMed ID: 9482715
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|