Pseudomonas aeruginosa PAO1, PA2965 (fabF1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2965
Name
fabF1
Replicon chromosome
Genomic location 3323574 - 3324818 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251655.1
GI 15598161
Affymetrix PA2965_fabF1_at
Entrez 879894
GenBank AAG06353.1
INSDC AAG06353.1
NCBI Locus Tag PA2965
protein_id(GenBank) gb|AAG06353.1|AE004722_9|gnl|PseudoCAP|PA2965
TIGR NTL03PA02965
UniParc UPI00000D428F
UniProtKB Acc G3XDA2
UniProtKB ID G3XDA2_PSEAE
UniRef100 UniRef100_G3XDA2
UniRef50 UniRef50_Q9KQH9
UniRef90 UniRef90_G3XDA2

Product

Feature Type CDS
Coding Frame 1
Product Name
beta-ketoacyl-acyl carrier protein synthase II
Product Name Confidence: Class 2
Synonyms FabF protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.74
Kyte-Doolittle Hydrophobicity Value -0.029
Molecular Weight (kDa) 43.5
Isoelectric Point (pI) 5.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7OC0 ANTIBIOTIC 04/25/21 Structure of Pseudomonas aeruginosa FabF mutant C164Q in complex with a ligand (2S,4R)-2-(thiophen-2-yl)thiazolidine-4-carboxylic acid Pseudomonas aeruginosa PAO1 1.78 X-RAY DIFFRACTION 99.8
4B7V TRANSFERASE 08/22/12 Structure of wild type Pseudomonas aeruginosa FabF (KASII) PSEUDOMONAS AERUGINOSA 1.73 X-RAY DIFFRACTION 100.0
4JB6 TRANSFERASE 02/19/13 Structure of Pseudomonas aeruginosa FabF mutant C164Q Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 99.8
4JPF TRANSFERASE 03/19/13 Structure of wild type Pseudomonas aeruginosa FabF (KASII) in Complex with ligand Pseudomonas aeruginosa 1.67 X-RAY DIFFRACTION 100.0
7OC1 ANTIBIOTIC 04/25/21 Structure of Pseudomonas aeruginosa FabF mutant C164Q in complex with Platensimycin Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 572 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002617 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2965
Search term: fabF1

Human Homologs

Ensembl 110, assembly GRCh38.p14
3-oxoacyl-ACP synthase, mitochondrial [Source:HGNC Symbol;Acc:HGNC:26063]
E-value: 3.2e-97
Percent Identity: 47.4

References

Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis.
Garwin JL, Klages AL, Cronan JE Jr
J Biol Chem 1980 Apr 25;255(8):3263-5
PubMed ID: 6988423
Cloning of the fabF gene in an expression vector and in vitro characterization of recombinant fabF and fabB encoded enzymes from Escherichia coli.
Edwards P, Nelsen JS, Metz JG, Dehesh K
FEBS Lett 1997 Jan 27;402(1):62-6
PubMed ID: 9013860
Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes.
Huang W, Jia J, Edwards P, Dehesh K, Schneider G, Lindqvist Y
EMBO J 1998 Mar 2;17(5):1183-91
PubMed ID: 9482715
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994