Pseudomonas aeruginosa PAO1, PA2968 (fabD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2968
Name
fabD
Replicon chromosome
Genomic location 3326145 - 3327083 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251658.1
GI 15598164
Affymetrix PA2968_fabD_at
DNASU PaCD00007295
Entrez 880434
GenBank AAG06356.1
INSDC AAG06356.1
NCBI Locus Tag PA2968
protein_id(GenBank) gb|AAG06356.1|AE004722_12|gnl|PseudoCAP|PA2968
TIGR NTL03PA02968
UniParc UPI00000D4230
UniProtKB Acc G3XCZ6
UniProtKB ID G3XCZ6_PSEAE
UniRef100 UniRef100_G3XCZ6
UniRef50 UniRef50_O85140
UniRef90 UniRef90_G3XCZ6

Product

Feature Type CDS
Coding Frame 1
Product Name
malonyl-CoA-[acyl-carrier-protein] transacylase
Product Name Confidence: Class 1
Synonyms FabD protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.42
Kyte-Doolittle Hydrophobicity Value 0.253
Molecular Weight (kDa) 32.4
Isoelectric Point (pI) 4.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 572 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002614 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2968
Search term: fabD

Human Homologs

Ensembl 110, assembly GRCh38.p14
malonyl-CoA-acyl carrier protein transacylase [Source:HGNC Symbol;Acc:HGNC:29622]
E-value: 1.5e-25
Percent Identity: 32.3

References

Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase.
Verwoert II, Verbree EC, van der Linden KH, Nijkamp HJ, Stuitje AR
J Bacteriol 1992 May;174(9):2851-7
PubMed ID: 1314802
Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD).
Kutchma AJ, Hoang TT, Schweizer HP
J Bacteriol 1999 Sep;181(17):5498-504
PubMed ID: 10464226
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994