Pseudomonas aeruginosa PAO1, PA2977 (murB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2977
Name
murB
Replicon chromosome
Genomic location 3336212 - 3337231 (- strand)

Cross-References

RefSeq NP_251667.1
GI 15598173
Affymetrix PA2977_murB_at
Entrez 880517
GenBank AAG06365.1
INSDC AAG06365.1
NCBI Locus Tag PA2977
protein_id(GenBank) gb|AAG06365.1|AE004723_9|gnl|PseudoCAP|PA2977
TIGR NTL03PA02977
UniParc UPI000012F93F
UniProtKB Acc Q9HZM7
UniProtKB ID MURB_PSEAE
UniRef100 UniRef100_Q9HZM7
UniRef50 UniRef50_Q9HZM7
UniRef90 UniRef90_Q9HZM7

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylpyruvoylglucosamine reductase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.87
Kyte-Doolittle Hydrophobicity Value -0.258
Molecular Weight (kDa) 37.6
Isoelectric Point (pI) 6.69

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7ORZ OXIDOREDUCTASE 06/06/21 Crystal structure of UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) from Pseudomonas aeruginosa in complex with FAD and a pyrazole derivative (fragment 18) Pseudomonas aeruginosa PAO1 1.85 X-RAY DIFFRACTION 100.0
7OR2 OXIDOREDUCTASE 06/04/21 Crystal structure of UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) from Pseudomonas aeruginosa in complex with FAD and a pyrazole derivative (fragment 4) Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.35 X-RAY DIFFRACTION 100.0
4JB1 OXIDOREDUCTASE 02/19/13 Crystal structure of P. aeruginosa MurB in complex with NADP+ Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0
7OSQ OXIDOREDUCTASE 06/09/21 Crystal structure of UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) from Pseudomonas aeruginosa in complex with FAD and a pyrazole derivative (fragment 18) Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.07 X-RAY DIFFRACTION 100.0
4JAY OXIDOREDUCTASE 02/19/13 Crystal structure of P. aeruginosa MurB in complex with NADP+ Pseudomonas aeruginosa 2.23 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 548 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002605 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2977
Search term: murB

Human Homologs

References

Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase.
Pucci MJ, Discotto LF, Dougherty TJ
J Bacteriol 1992 Mar;174(5):1690-3
PubMed ID: 1311302