Pseudomonas aeruginosa PAO1, PA3013 (faoB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3013
Name	faoB Synonym: fadA Synonym: fadA5
Replicon	chromosome
Genomic location	3373254 - 3374429 (- strand)
Transposon Mutants	4 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	PsrA binds to the promoter region of faoAB. PsrA also binds to and responds to long chain fatty acids to derepress the faoAB operon.

Cross-References

RefSeq	NP_251703.1
GI	15598209
Affymetrix	PA3013_foaB_at
Entrez	880523
GenBank	AAG06401.1
INSDC	AAG06401.1
NCBI Locus Tag	PA3013
protein_id(GenBank)	gb|AAG06401.1|AE004726_7|gnl|PseudoCAP|PA3013
TIGR	NTL03PA03013
UniParc	UPI00000C58C8
UniProtKB Acc	Q9HZJ3
UniProtKB ID	FADA_PSEAE
UniRef100	UniRef100_Q9HZJ3
UniRef50	UniRef50_P21151
UniRef90	UniRef90_P28790

Product

Feature Type	CDS
Coding Frame	1
Product Name	fatty-acid oxidation complex beta-subunit Product Name Confidence: Class 2
Synonyms	3-ketoacyl-coA thiolase
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-1.26
Kyte-Doolittle Hydrophobicity Value	-0.049
Molecular Weight (kDa)	41.6
Isoelectric Point (pI)	6.79

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 1 Cytoplasmic Class 2 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
1WDM	LYASE,OXIDOREDUCTASE/TRANSFERASE	05/17/04	fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form I (native3)	Pseudomonas fragi	3.8	X-RAY DIFFRACTION	90.5
1WDL	LYASE,OXIDOREDUCTASE/TRANSFERASE	05/17/04	fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form II (native4)	Pseudomonas fragi	3.5	X-RAY DIFFRACTION	90.5
2D3T	LYASE, OXIDOREDUCTASE/TRANSFERASE	10/01/05	Fatty Acid beta-oxidation multienzyme complex from Pseudomonas Fragi, Form V	Pseudomonas fragi	3.4	X-RAY DIFFRACTION	90.5
1WDK	LYASE,OXIDOREDUCTASE/TRANSFERASE	05/17/04	fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form I (native2)	Pseudomonas fragi	2.5	X-RAY DIFFRACTION	90.5

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 478 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002569 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3013 Search term: faoB Search term: fatty-acid oxidation complex beta-subunit

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000333664 acetyl-CoA acyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:82] E-value: 3.2e-62 Percent Identity: 40.4

References

Primary structures of the genes, faoA and faoB, from Pseudomonas fragi B-0771 which encode the two subunits of the HDT multienzyme complex involved in fatty acid beta-oxidation. Sato S, Hayashi M, Imamura S, Ozeki Y, Kawaguchi A J Biochem 1992 Jan;111(1):8-15 PubMed ID: 1607366