Pseudomonas aeruginosa PAO1, PA3108 (purF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3108
Name
purF
Replicon chromosome
Genomic location 3488409 - 3489914 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_251798.1
GI 15598304
Affymetrix PA3108_purF_at
Entrez 880477
GenBank AAG06496.1
INSDC AAA83434.1
NCBI Locus Tag PA3108
protein_id(GenBank) gb|AAG06496.1|AE004735_3|gnl|PseudoCAP|PA3108
TIGR NTL03PA03108
UniParc UPI0000165ECD
UniProtKB Acc Q51342
UniProtKB ID PUR1_PSEAE
UniRef100 UniRef100_Q51342
UniRef50 UniRef50_Q51342
UniRef90 UniRef90_Q51342

Product

Feature Type CDS
Coding Frame 1
Product Name
amidophosphoribosyltransferase
Product Name Confidence: Class 2
Synonyms glutamine phosphoribosylpyrophosphate amidotransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (55.37 kDa, PMID:21751344)
Charge (pH 7) -1.66
Kyte-Doolittle Hydrophobicity Value -0.175
Molecular Weight (kDa) 55.4
Isoelectric Point (pI) 6.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 652 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002490 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3108
Search term: purF

Human Homologs

Ensembl 110, assembly GRCh38.p14
phosphoribosyl pyrophosphate amidotransferase [Source:HGNC Symbol;Acc:HGNC:9238]
E-value: 5.7e-64
Percent Identity: 33.8

References

Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site, and trace metal analysis.
Tso JY, Hermodson MA, Zalkin H
J Biol Chem 1982 Apr 10;257(7):3532-6
PubMed ID: 7037784
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344