Pseudomonas aeruginosa PAO1, PA3118 (leuB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3118
Name
leuB
Replicon chromosome
Genomic location 3500668 - 3501750 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251808.1
GI 15598314
Affymetrix PA3118_leuB_at
DNASU PaCD00007371
Entrez 882812
GenBank AAG06506.1
INSDC AAG06506.1
NCBI Locus Tag PA3118
protein_id(GenBank) gb|AAG06506.1|AE004736_3|gnl|PseudoCAP|PA3118
TIGR NTL03PA03118
UniParc UPI000012E55B
UniProtKB Acc Q51375
UniProtKB ID LEU3_PSEAE
UniRef100 UniRef100_Q51375
UniRef50 UniRef50_Q56268
UniRef90 UniRef90_Q51375

Product

Feature Type CDS
Coding Frame 1
Product Name
3-isopropylmalate dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.36
Kyte-Doolittle Hydrophobicity Value -0.067
Molecular Weight (kDa) 39.1
Isoelectric Point (pI) 4.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 605 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002480 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3118
Search term: leuB

Human Homologs

Ensembl 110, assembly GRCh38.p14
isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit beta [Source:HGNC Symbol;Acc:HGNC:5385]
E-value: 1.8e-27
Percent Identity: 29.2

References

Characterization and mutagenesis of the leucine biosynthetic genes of Azotobacter vinelandii: an analysis of the rarity of amino acid auxotrophs.
Manna AC, Das HK
Mol Gen Genet 1997 Mar 26;254(2):207-17
PubMed ID: 9108283