Pseudomonas aeruginosa PAO1, PA3133 (sawR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3133
Name
sawR
Replicon chromosome
Genomic location 3514023 - 3514565 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentSawR down-regulates the expression levels of hmgA and this causes the accumulation of the brown pigment pyomelanin. Note that SawR does not bind directly to the hmgA promoter.
CommentsawR is highly overexpressed when P. aeruginosa is exposed to Surface Acoustic Waves.

Cross-References

RefSeq NP_251823.1
GI 15598329
Affymetrix PA3133_at
DNASU PaCD00007377
Entrez 880097
GenBank AAG06521.1
INSDC AAG06521.1
NCBI Locus Tag PA3133
protein_id(GenBank) gb|AAG06521.1|AE004737_7|gnl|PseudoCAP|PA3133
TIGR NTL03PA03133
UniParc UPI00000C5922
UniProtKB Acc Q9HZ91
UniProtKB ID Q9HZ91_PSEAE
UniRef100 UniRef100_Q9HZ91
UniRef50 UniRef50_Q9HZ91
UniRef90 UniRef90_Q9HZ91

Product

Feature Type CDS
Coding Frame 1
Product Name
SawR
Product Name Confidence: Class 1
Synonyms probable transcriptional regulator
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.31
Kyte-Doolittle Hydrophobicity Value 0.008
Molecular Weight (kDa) 19.2
Isoelectric Point (pI) 6.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2FD5 TRANSCRIPTION REGULATOR 12/13/05 The crystal structure of a transcriptional regulator from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 154 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002465 (448 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3133
Search term: sawR
Search term: SawR

Human Homologs

References

Surface acoustic waves increase the susceptibility of Pseudomonas aeruginosa biofilms to antibiotic treatment.
Kopel M, Degtyar E, Banin E
Biofouling 2011 Aug;27(7):701-10
PubMed ID: 21732715
SawR a new regulator controlling pyomelanin synthesis in Pseudomonas aeruginosa.
Ben-David Y, Zlotnik E, Zander I, Yerushalmi G, Shoshani S, Banin E
Microbiol Res 2018 Jan;206:91-98
PubMed ID: 29146265