Pseudomonas aeruginosa PAO1, PA3195 (gapA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3195
Name
gapA
Replicon chromosome
Genomic location 3587433 - 3588437 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251885.1
GI 15598391
Affymetrix PA3195_gapA_at
Entrez 882829
GenBank AAG06583.1
INSDC AAG06583.1
NCBI Locus Tag PA3195
protein_id(GenBank) gb|AAG06583.1|AE004743_6|gnl|PseudoCAP|PA3195
TIGR NTL03PA03195
UniParc UPI000012AED1
UniProtKB Acc P27726
UniProtKB ID G3P_PSEAE
UniRef100 UniRef100_P27726
UniRef50 UniRef50_Q3ILL8
UniRef90 UniRef90_P27726

Product

Feature Type CDS
Coding Frame 1
Product Name
glyceraldehyde 3-phosphate dehydrogenase
Product Name Confidence: Class 1
Synonyms NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
GAPDH
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.73
Kyte-Doolittle Hydrophobicity Value -0.028
Molecular Weight (kDa) 36.2
Isoelectric Point (pI) 6.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 607 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002421 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3195
Search term: gapA

Human Homologs

Ensembl 110, assembly GRCh38.p14
glyceraldehyde-3-phosphate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4141]
E-value: 4.3e-76
Percent Identity: 46.4
Ensembl 110, assembly GRCh38.p14
glyceraldehyde-3-phosphate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4141]
E-value: 4.3e-76
Percent Identity: 46.4
Ensembl 110, assembly GRCh38.p14
glyceraldehyde-3-phosphate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4141]
E-value: 4.3e-76
Percent Identity: 46.4

References

Two genes for carbohydrate catabolism are divergently transcribed from a region of DNA containing the hexC locus in Pseudomonas aeruginosa PAO1.
Temple L, Sage A, Christie GE, Phibbs PV Jr
J Bacteriol 1994 Aug;176(15):4700-9
PubMed ID: 8045900