Pseudomonas aeruginosa PAO1, PA3279 (oprP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3279
Name
oprP
Replicon chromosome
Genomic location 3671227 - 3672549 (- strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251969.1
GI 15598475
Affymetrix PA3279_oprP_at
Entrez 882442
GenBank AAG06667.1
INSDC CAA68631.1
NCBI Locus Tag PA3279
protein_id(GenBank) gb|AAG06667.1|AE004750_5|gnl|PseudoCAP|PA3279
TIGR NTL03PA03279
UniParc UPI0000131F7F
UniProtKB Acc P05695
UniProtKB ID PORP_PSEAE
UniRef100 UniRef100_P05695
UniRef50 UniRef50_P05695
UniRef90 UniRef90_P05695

Product

Feature Type CDS
Coding Frame 1
Product Name
Phosphate-specific outer membrane porin OprP precursor
Product Name Confidence: Class 1
Synonyms porin P
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.06
Kyte-Doolittle Hydrophobicity Value -0.453
Molecular Weight (kDa) 48.2
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2O4V MEMBRANE PROTEIN 12/05/06 An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane Pseudomonas aeruginosa PAO1 1.94 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
(Hydroxyethyloxy)Tri(Ethyloxy)Octane DB04233 DrugBank 5.1.4 P05695 Porin P 100.0 440 3.7e-256

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 49 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002334 (687 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3279
Search term: oprP

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Demonstration and chemical modification of a specific phosphate binding site in the phosphate-starvation-inducible outer membrane porin protein P of Pseudomonas aeruginosa.
Hancock RE, Benz R
Biochim Biophys Acta 1986 Sep 11;860(3):699-707
PubMed ID: 3017428