Pseudomonas aeruginosa PAO1, PA3286

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3286
Name
Replicon chromosome
Genomic location 3678334 - 3679386 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPA3286 is also a beta-acetoacetyl-acyl carrier protein synthase. It belongs to the KASIII family (like FabH), but has a unique preference for octanoyl-CoA allowing it to shunt exogenous fatty acids from fatty acid beta-oxidation directly into fatty acid synthesis. Despite this specificity it does retains sufficient activity with acetyl-CoA to cover for FabY in initiating fatty acid synthesis. PA3286 is synthetically lethal with PA5174.

Cross-References

RefSeq NP_251976.1
GI 15598482
Affymetrix PA3286_at
Entrez 882449
GenBank AAG06674.1
INSDC AAG06674.1
NCBI Locus Tag PA3286
protein_id(GenBank) gb|AAG06674.1|AE004750_12|gnl|PseudoCAP|PA3286
TIGR NTL03PA03286
UniParc UPI00000C5992
UniProtKB Acc Q9HYV7
UniProtKB ID BEKAS_PSEAE
UniRef100 UniRef100_Q9HYV7
UniRef50 UniRef50_Q9HYV7
UniRef90 UniRef90_Q9HYV7

Product

Feature Type CDS
Coding Frame 1
Product Name
beta-acetoacetyl-acyl carrier protein synthase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Detected by iTRAQ (PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.77
Kyte-Doolittle Hydrophobicity Value -0.039
Molecular Weight (kDa) 38.1
Isoelectric Point (pI) 6.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4266
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 539 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002327 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3286

Human Homologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Pseudomonas aeruginosa directly shunts β-oxidation degradation intermediates into de novo fatty acid biosynthesis.
Yuan Y, Leeds JA, Meredith TC
J Bacteriol 2012 Oct;194(19):5185-96
PubMed ID: 22753057
Will the initiator of fatty acid synthesis in Pseudomonas aeruginosa please stand up?
Zhang YM, Rock CO
J Bacteriol 2012 Oct;194(19):5159-61
PubMed ID: 22821980