Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3286
|
Name |
|
Replicon | chromosome |
Genomic location | 3678334 - 3679386 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Comment | PA3286 is also a beta-acetoacetyl-acyl carrier protein synthase. It belongs to the KASIII family (like FabH), but has a unique preference for octanoyl-CoA allowing it to shunt exogenous fatty acids from fatty acid beta-oxidation directly into fatty acid synthesis. Despite this specificity it does retains sufficient activity with acetyl-CoA to cover for FabY in initiating fatty acid synthesis. PA3286 is synthetically lethal with PA5174. |
RefSeq | NP_251976.1 |
GI | 15598482 |
Affymetrix | PA3286_at |
Entrez | 882449 |
GenBank | AAG06674.1 |
INSDC | AAG06674.1 |
NCBI Locus Tag | PA3286 |
protein_id(GenBank) | gb|AAG06674.1|AE004750_12|gnl|PseudoCAP|PA3286 |
TIGR | NTL03PA03286 |
UniParc | UPI00000C5992 |
UniProtKB Acc | Q9HYV7 |
UniProtKB ID | BEKAS_PSEAE |
UniRef100 | UniRef100_Q9HYV7 |
UniRef50 | UniRef50_Q9HYV7 |
UniRef90 | UniRef90_Q9HYV7 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
beta-acetoacetyl-acyl carrier protein synthase
|
Synonyms | |
Evidence for Translation |
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Detected by iTRAQ (PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -3.77 |
Kyte-Doolittle Hydrophobicity Value | -0.039 |
Molecular Weight (kDa) | 38.1 |
Isoelectric Point (pI) | 6.35 |
Individual Mappings | |
Additional evidence for subcellular localization |
Source 1 | |
Database | Victors : 4266 |
Category | |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Rattus norvegicus |
Notes | |
Infection model | Rat agar bead model of chronic lung infection |
Reference | 14641575 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 539 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002327 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3286
Search term: beta-acetoacetyl-acyl carrier protein synthase
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Pseudomonas aeruginosa directly shunts β-oxidation degradation intermediates into de novo fatty acid biosynthesis.
Yuan Y, Leeds JA, Meredith TC
J Bacteriol 2012 Oct;194(19):5185-96
PubMed ID: 22753057
|
Will the initiator of fatty acid synthesis in Pseudomonas aeruginosa please stand up?
Zhang YM, Rock CO
J Bacteriol 2012 Oct;194(19):5159-61
PubMed ID: 22821980
|