Pseudomonas aeruginosa PAO1, PA3319 (plcN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3319
Name
plcN
Replicon chromosome
Genomic location 3720681 - 3722759 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252009.1
GI 15598515
Affymetrix PA3319_plcN_at
Entrez 882484
GenBank AAG06707.1
INSDC AAG06707.1
NCBI Locus Tag PA3319
protein_id(GenBank) gb|AAG06707.1|AE004754_3|gnl|PseudoCAP|PA3319
TIGR NTL03PA03319
UniParc UPI0000131987
UniProtKB Acc P15713
UniProtKB ID PHLN_PSEAE
UniRef100 UniRef100_P15713
UniRef50 UniRef50_P15713
UniRef90 UniRef90_P15713

Product

Feature Type CDS
Coding Frame 1
Product Name
non-hemolytic phospholipase C precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 13.50
Kyte-Doolittle Hydrophobicity Value -0.510
Molecular Weight (kDa) 77.1
Isoelectric Point (pI) 9.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB
Category Non-hemolytic phospholipase C
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 98 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002303 (317 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3319
Search term: plcN

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa.
Ostroff RM, Vasil AI, Vasil ML
J Bacteriol 1990 Oct;172(10):5915-23
PubMed ID: 2120196
Involvement of the twin-arginine translocation system in protein secretion via the type II pathway.
Voulhoux R, Ball G, Ize B, Vasil ML, Lazdunski A, Wu LF, Filloux A
EMBO J 2001 Dec 3;20(23):6735-41
PubMed ID: 11726509