Pseudomonas aeruginosa PAO1, PA3348 (cheR1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3348
Name
cheR1
Synonym: cheR1
Replicon chromosome
Genomic location 3759996 - 3760820 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252038.1
GI 15598544
Affymetrix PA3348_at
DNASU PaCD00007466
Entrez 882515
GenBank AAG06736.1
INSDC AAG06736.1
NCBI Locus Tag PA3348
protein_id(GenBank) gb|AAG06736.1|AE004757_5|gnl|PseudoCAP|PA3348
TIGR NTL03PA03348
UniParc UPI00001278B8
UniProtKB Acc O87131
UniProtKB ID CHER1_PSEAE
UniRef100 UniRef100_O87131
UniRef50 UniRef50_Q88ER1
UniRef90 UniRef90_O87131

Product

Feature Type CDS
Coding Frame 1
Product Name
CheR1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.35
Kyte-Doolittle Hydrophobicity Value -0.132
Molecular Weight (kDa) 30.6
Isoelectric Point (pI) 9.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5Y4S TRANSFERASE 08/04/17 Structure of a methyltransferase complex Pseudomonas aeruginosa str. PAO1 3.405 X-RAY DIFFRACTION 100.0
5Y4R TRANSFERASE/PROTEIN BINDING 08/04/17 Structure of a methyltransferase complex Pseudomonas aeruginosa str. PAO1 2.298 X-RAY DIFFRACTION 100.0
5XLY TRANSFERASE 05/12/17 Crystal structure of CheR1 in complex with c-di-GMP-bound MapZ Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.763 X-RAY DIFFRACTION 100.0
5XLX TRANSFERASE 05/12/17 Crystal structure of the C-terminal domain of CheR1 containing SAH Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.969 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 414 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002280 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3348
Search term: cheR1
Search term: CheR1

Human Homologs

References

Chemotactic motility is required for invasion of the host by the fish pathogen Vibrio anguillarum.
O'Toole R, Milton DL, Wolf-Watz H
Mol Microbiol 1996 Feb;19(3):625-37
PubMed ID: 8830252
Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa.
Kato J, Nakamura T, Kuroda A, Ohtake H
Biosci Biotechnol Biochem 1999 Jan;63(1):155-61
PubMed ID: 10052136
The Pseudomonas aeruginosa chemotaxis methyltransferase CheR1 impacts on bacterial surface sampling.
Schmidt J, Müsken M, Becker T, Magnowska Z, Bertinetti D, Möller S, Zimmermann B, Herberg FW, Jänsch L, Häussler S
PLoS One 2011 Mar 22;6(3):e18184
PubMed ID: 21445368