Pseudomonas aeruginosa PAO1, PA3385 (amrZ)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: algZ
Replicon chromosome
Genomic location 3791347 - 3791673 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_252075.1
GI 15598581
Affymetrix PA3385_at
DNASU PaCD00007486
Entrez 878714
GenBank AAG06773.1
INSDC AAG06773.1
NCBI Locus Tag PA3385
protein_id(GenBank) gb|AAG06773.1|AE004760_1|gnl|PseudoCAP|PA3385
UniParc UPI00000D4234
UniProtKB Acc G3XCY4
UniRef100 UniRef100_W0YXA2
UniRef50 UniRef50_F8H3I4
UniRef90 UniRef90_W0YXA2


Feature Type CDS
Coding Frame 1
Product Name
alginate and motility regulator Z
Product Name Confidence: Class 1
Synonyms AlgZ
Evidence for Translation
Charge (pH 7) 1.19
Kyte-Doolittle Hydrophobicity Value -0.534
Molecular Weight (kDa) 12.3
Isoelectric Point (pI) 7.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3QOQ Transcription/DNA 02/10/11 Crystal Structure of the Transcription Factor AmrZ in Complex with the 18 Base Pair amrZ1 Binding Site PSEUDOMONAS AERUGINOSA; SYNTHETIC CONSTRUCT 3.1 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002243 (544 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3385
Search term: amrZ


The Pseudomonas aeruginosa ribbon-helix-helix DNA-binding protein AlgZ (AmrZ) controls twitching motility and biogenesis of type IV pili.
Baynham PJ, Ramsey DM, Gvozdyev BV, Cordonnier EM, Wozniak DJ
J Bacteriol 2006 Jan;188(1):132-40
PubMed ID: 16352829
Binding of Pseudomonas aeruginosa AlgZ to sites upstream of the algZ promoter leads to repression of transcription.
Ramsey DM, Baynham PJ, Wozniak DJ
J Bacteriol 2005 Jul;187(13):4430-43
PubMed ID: 15968052
Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT.
Wozniak DJ, Sprinkle AB, Baynham PJ
J Bacteriol 2003 Dec;185(24):7297-300
PubMed ID: 14645293
Pseudomonas aeruginosa AlgZ, a ribbon-helix-helix DNA-binding protein, is essential for alginate synthesis and algD transcriptional activation.
Baynham PJ, Brown AL, Hall LL, Wozniak DJ
Mol Microbiol 1999 Sep;33(5):1069-80
PubMed ID: 10476040
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999