Pseudomonas aeruginosa PAO1, PA3452 (mqoA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3452
Name
mqoA
Replicon chromosome
Genomic location 3856749 - 3858320 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252142.1
GI 15598648
Affymetrix PA3452_mqoA_at
Entrez 879029
GenBank AAG06840.1
INSDC AAG06840.1
NCBI Locus Tag PA3452
protein_id(GenBank) gb|AAG06840.1|AE004766_1|gnl|PseudoCAP|PA3452
TIGR NTL03PA03452
UniParc UPI000012F4BE
UniProtKB Acc Q9HYF4
UniProtKB ID MQO1_PSEAE
UniRef100 UniRef100_Q9HYF4
UniRef50 UniRef50_P33940
UniRef90 UniRef90_Q9HYF4

Product

Feature Type CDS
Coding Frame 1
Product Name
malate:quinone oxidoreductase
Product Name Confidence: Class 2
Synonyms malate dehydrogenase
Evidence for Translation
Detected using LC-MS/MS (57.21 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.14
Kyte-Doolittle Hydrophobicity Value -0.200
Molecular Weight (kDa) 57.2
Isoelectric Point (pI) 5.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 156 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002188 (502 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3452
Search term: mqoA

Human Homologs

References

A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T
DNA Res 1996 Dec 31;3(6):379-92
PubMed ID: 9097040
Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum.
Molenaar D, van der Rest ME, Petrović S
Eur. J. Biochem. 1998 Jun;254(2):395-403
PubMed ID: 9660197
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344