Pseudomonas aeruginosa PAO1, PA3471

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3471
Name
Synonym: sfcA
Replicon chromosome
Genomic location 3883560 - 3885254 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252161.1
GI 15598667
Affymetrix PA3471_at
Entrez 879925
GenBank AAG06859.1
INSDC AAG06859.1
NCBI Locus Tag PA3471
protein_id(GenBank) gb|AAG06859.1|AE004768_4|gnl|PseudoCAP|PA3471
TIGR NTL03PA03471
UniParc UPI00000C5A2D
UniProtKB Acc Q9HYD5
UniProtKB ID MAO1_PSEAE
UniRef100 UniRef100_Q9HYD5
UniRef50 UniRef50_Q9HYD5
UniRef90 UniRef90_Q9HYD5

Product

Feature Type CDS
Coding Frame 1
Product Name
probable malic enzyme
Product Name Confidence: Class 3
Synonyms probable malate dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by iTRAQ (PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -11.87
Kyte-Doolittle Hydrophobicity Value -0.154
Molecular Weight (kDa) 62.4
Isoelectric Point (pI) 5.18

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 484 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002170 (324 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3471

Human Homologs

Ensembl 110, assembly GRCh38.p14
malic enzyme 1 [Source:HGNC Symbol;Acc:HGNC:6983]
E-value: 4.0e-122
Percent Identity: 42.2

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142