Pseudomonas aeruginosa PAO1, PA3482 (metG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3482
Name
metG
Replicon chromosome
Genomic location 3895324 - 3897357 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252172.1
GI 15598678
Affymetrix PA3482_metG_at
Entrez 878831
GenBank AAG06870.1
INSDC AAG06870.1
NCBI Locus Tag PA3482
protein_id(GenBank) gb|AAG06870.1|AE004769_3|gnl|PseudoCAP|PA3482
TIGR NTL03PA03482
UniParc UPI00001365AA
UniProtKB Acc Q9HYC7
UniProtKB ID SYM_PSEAE
UniRef100 UniRef100_Q9HYC7
UniRef50 UniRef50_Q9HYC7
UniRef90 UniRef90_Q9HYC7

Product

Feature Type CDS
Coding Frame 1
Product Name
methionyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -14.63
Kyte-Doolittle Hydrophobicity Value -0.192
Molecular Weight (kDa) 74.9
Isoelectric Point (pI) 5.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7ULZ LIGASE 04/05/22 Crystal Structure of Methionine-tRNA ligase / Methionyl-tRNA synthetase (MetRS) from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002160 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3482
Search term: metG

Human Homologs

Ensembl 110, assembly GRCh38.p14
methionyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:25133]
E-value: 6.8e-36
Percent Identity: 25.4

References

Methionyl-tRNA synthetase from E. coli--a review.
Meinnel T, Mechulam Y, Dardel F, Schmitter JM, Hountondji C, Brunie S, Dessen P, Fayat G, Blanquet S
Biochimie 1990 Aug;72(8):625-32
PubMed ID: 2126467
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994