Pseudomonas aeruginosa PAO1, PA3525 (argG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3525
Name
argG
Replicon chromosome
Genomic location 3944662 - 3945879 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252215.1
GI 15598721
Affymetrix PA3525_argG_at
Entrez 878872
GenBank AAG06913.1
INSDC AAG06913.1
NCBI Locus Tag PA3525
protein_id(GenBank) gb|AAG06913.1|AE004773_2|gnl|PseudoCAP|PA3525
TIGR NTL03PA03525
UniParc UPI000012618F
UniProtKB Acc Q9HY84
UniProtKB ID ASSY_PSEAE
UniRef100 UniRef100_B7UVV5
UniRef50 UniRef50_A6WV13
UniRef90 UniRef90_Q87XM3

Product

Feature Type CDS
Coding Frame 1
Product Name
argininosuccinate synthase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -8.36
Kyte-Doolittle Hydrophobicity Value -0.319
Molecular Weight (kDa) 45.3
Isoelectric Point (pI) 5.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 589 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002140 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3525
Search term: argG

Human Homologs

Ensembl 110, assembly GRCh38.p14
argininosuccinate synthase 1 [Source:HGNC Symbol;Acc:HGNC:758]
E-value: 1.5e-102
Percent Identity: 47.3
Ensembl 110, assembly GRCh38.p14
argininosuccinate synthase 1 [Source:HGNC Symbol;Acc:HGNC:758]
E-value: 1.5e-102
Percent Identity: 47.3
Ensembl 110, assembly GRCh38.p14
argininosuccinate synthase 1 [Source:HGNC Symbol;Acc:HGNC:758]
E-value: 1.5e-102
Percent Identity: 47.3

References

Characterization of human wild-type and mutant argininosuccinate synthetase proteins expressed in bacterial cells.
Shaheen N, Kobayashi K, Terazono H, Fukushige T, Horiuchi M, Saheki T
Enzyme Protein 1994-1995;48(5-6):251-64
PubMed ID: 8792870
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994