Pseudomonas aeruginosa PAO1, PA3528 (rnt)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3528
Name
rnt
Replicon chromosome
Genomic location 3948138 - 3948812 (+ strand)

Cross-References

RefSeq NP_252218.1
GI 15598724
Affymetrix PA3528_rnt_at
Entrez 879810
GenBank AAG06916.1
INSDC AAG06916.1
NCBI Locus Tag PA3528
protein_id(GenBank) gb|AAG06916.1|AE004773_5|gnl|PseudoCAP|PA3528
TIGR NTL03PA03528
UniParc UPI00001344FB
UniProtKB Acc Q9HY82
UniProtKB ID RNT_PSEAE
UniRef100 UniRef100_Q02QZ9
UniRef50 UniRef50_P30014
UniRef90 UniRef90_A6V1R8

Product

Feature Type CDS
Coding Frame 1
Product Name
ribonuclease T
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.64
Kyte-Doolittle Hydrophobicity Value -0.262
Molecular Weight (kDa) 24.7
Isoelectric Point (pI) 4.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2F96 Structural genomics, Hydrolase 12/05/05 2.1 A crystal structure of Pseudomonas aeruginosa rnase T (Ribonuclease T) Pseudomonas aeruginosa 2.09 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 153 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002137 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3528
Search term: rnt
Search term: ribonuclease T

Human Homologs

References

RNase T affects Escherichia coli growth and recovery from metabolic stress.
Padmanabha KP, Deutscher MP
J Bacteriol 1991 Feb;173(4):1376-81
PubMed ID: 1704882
Sequence and transcriptional analysis of the Escherichia coli rnt gene encoding RNase T.
Huang S, Deutscher MP
J Biol Chem 1992 Dec 15;267(35):25609-13
PubMed ID: 1460056
Identification of a potent DNase activity associated with RNase T of Escherichia coli.
Viswanathan M, Dower KW, Lovett ST
J Biol Chem 1998 Dec 25;273(52):35126-31
PubMed ID: 9857048