Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3540
|
Name |
algD
|
Replicon | chromosome |
Genomic location | 3962825 - 3964135 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252230.1 |
GI | 15598736 |
Affymetrix | PA3540_algD_at |
Entrez | 879004 |
GenBank | AAG06928.1 |
INSDC | AAC36874.1 |
NCBI Locus Tag | PA3540 |
protein_id(GenBank) | gb|AAG06928.1|AE004774_6|gnl|PseudoCAP|PA3540 |
TIGR | NTL03PA03540 |
UniParc | UPI000012584C |
UniProtKB Acc | P11759 |
UniProtKB ID | ALGD_PSEAE |
UniRef100 | UniRef100_P11759 |
UniRef50 | UniRef50_P11759 |
UniRef90 | UniRef90_P11759 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
GDP-mannose 6-dehydrogenase AlgD
|
Synonyms | |
Evidence for Translation | |
Charge (pH 7) | -9.09 |
Kyte-Doolittle Hydrophobicity Value | -0.051 |
Molecular Weight (kDa) | 47.6 |
Isoelectric Point (pI) | 5.35 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1MFZ | OXIDOREDUCTASE | 08/14/02 | Partially refined 2.8 A Crystal structure of GDP-mannose dehydrogenase from P. aeruginosa | Pseudomonas aeruginosa | 2.8 | X-RAY DIFFRACTION | 100.0 |
1MV8 | OXIDOREDUCTASE | 09/24/02 | 1.55 A crystal structure of a ternary complex of GDP-mannose dehydrogenase from Psuedomonas aeruginosa | Pseudomonas aeruginosa | 1.55 | X-RAY DIFFRACTION | 100.0 |
1MUU | OXIDOREDUCTASE | 09/24/02 | 2.0 A crystal structure of GDP-mannose dehydrogenase | Pseudomonas aeruginosa | 2.02 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Guanosine 5'-(Trihydrogen Diphosphate), P'-D-Mannopyranosyl Ester | DB04023 | DrugBank | 5.1.4 | P11759 | GDP-mannose 6-dehydrogenase | 100.0 | 436 | 7.2e-252 |
Sucrose | DB02772 | DrugBank | 5.1.4 | P11759 | GDP-mannose 6-dehydrogenase | 100.0 | 436 | 7.2e-252 |
Source 1 | |
Database | VFDB : VF0091 |
Category | Alginate biosynthesis |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002126 (490 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3540
Search term: algD
Search term: GDP-mannose 6-dehydrogenase AlgD
|
Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa.
Franklin MJ, Ohman DE
J Bacteriol 2002 Jun;184(11):3000-7
PubMed ID: 12003941
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Pseudomonas aeruginosa infection in cystic fibrosis: nucleotide sequence and transcriptional regulation of the algD gene.
Deretic V, Gill JF, Chakrabarty AM
Nucleic Acids Res 1987 Jun 11;15(11):4567-81
PubMed ID: 3108855
|
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
Maharaj R, May TB, Wang SK, Chakrabarty AM
Gene 1993 Dec 22;136(1-2):267-9
PubMed ID: 8294014
|
GDP-mannose dehydrogenase is the key regulatory enzyme in alginate biosynthesis in Pseudomonas aeruginosa: evidence from metabolite studies.
Tatnell PJ, Russell NJ, Gacesa P
Microbiology (Reading) 1994 Jul;140 ( Pt 7):1745-54
PubMed ID: 7521247
|