Pseudomonas aeruginosa PAO1, PA3540 (algD)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 3962825 - 3964135 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_252230.1
GI 15598736
Affymetrix PA3540_algD_at
Entrez 879004
GenBank AAG06928.1
INSDC AAC36874.1
NCBI Locus Tag PA3540
protein_id(GenBank) gb|AAG06928.1|AE004774_6|gnl|PseudoCAP|PA3540
UniParc UPI000012584C
UniProtKB Acc P11759
UniRef100 UniRef100_P11759
UniRef50 UniRef50_P11759
UniRef90 UniRef90_P11759


Feature Type CDS
Coding Frame 1
Product Name
GDP-mannose 6-dehydrogenase AlgD
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -9.09
Kyte-Doolittle Hydrophobicity Value -0.051
Molecular Weight (kDa) 47.6
Isoelectric Point (pI) 5.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1MFZ OXIDOREDUCTASE 08/14/02 Partially refined 2.8 A Crystal structure of GDP-mannose dehydrogenase from P. aeruginosa PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 100.0
1MUU OXIDOREDUCTASE 09/24/02 2.0 A crystal structure of GDP-mannose dehydrogenase PSEUDOMONAS AERUGINOSA 2.02 X-RAY DIFFRACTION 100.0
1MV8 OXIDOREDUCTASE 09/24/02 1.55 A crystal structure of a ternary complex of GDP-mannose dehydrogenase from Psuedomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.55 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Guanosine 5'-(Trihydrogen Diphosphate), P'-D-Mannopyranosyl Ester DB04023 DrugBank 5.1.4 P11759 GDP-mannose 6-dehydrogenase 100.0 436 7.2e-252
Sucrose DB02772 DrugBank 5.1.4 P11759 GDP-mannose 6-dehydrogenase 100.0 436 7.2e-252

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Host Organism

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002126 (506 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3540
Search term: algD


Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa.
Franklin MJ, Ohman DE
J Bacteriol 2002 Jun;184(11):3000-7
PubMed ID: 12003941
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Pseudomonas aeruginosa infection in cystic fibrosis: nucleotide sequence and transcriptional regulation of the algD gene.
Deretic V, Gill JF, Chakrabarty AM
Nucleic Acids Res 1987 Jun 11;15(11):4567-81
PubMed ID: 3108855
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
Maharaj R, May TB, Wang SK, Chakrabarty AM
Gene 1993 Dec;136(1-2):267-9
PubMed ID: 8294014
GDP-mannose dehydrogenase is the key regulatory enzyme in alginate biosynthesis in Pseudomonas aeruginosa: evidence from metabolite studies.
Tatnell PJ, Russell NJ, Gacesa P
Microbiology (Reading, Engl.) 1994 Jul;140 ( Pt 7)
PubMed ID: 7521247