Pseudomonas aeruginosa PAO1, PA3540 (algD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA3540
Name
algD
Replicon chromosome
Genomic location 3962825 - 3964135 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252230.1
GI 15598736
Affymetrix PA3540_algD_at
Entrez 879004
GenBank AAG06928.1
INSDC AAC36874.1
NCBI Locus Tag PA3540
protein_id(GenBank) gb|AAG06928.1|AE004774_6|gnl|PseudoCAP|PA3540
TIGR NTL03PA03540
UniParc UPI000012584C
UniProtKB Acc P11759
UniProtKB ID ALGD_PSEAE
UniRef100 UniRef100_P11759
UniRef50 UniRef50_P11759
UniRef90 UniRef90_P11759

Product

Feature Type CDS
Coding Frame 1
Product Name
GDP-mannose 6-dehydrogenase AlgD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -9.09
Kyte-Doolittle Hydrophobicity Value -0.051
Molecular Weight (kDa) 47.6
Isoelectric Point (pI) 5.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1MV8 OXIDOREDUCTASE 09/24/02 1.55 A crystal structure of a ternary complex of GDP-mannose dehydrogenase from Psuedomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.55 X-RAY DIFFRACTION 100.0
1MUU OXIDOREDUCTASE 09/24/02 2.0 A crystal structure of GDP-mannose dehydrogenase PSEUDOMONAS AERUGINOSA 2.02 X-RAY DIFFRACTION 100.0
1MFZ OXIDOREDUCTASE 08/14/02 Partially refined 2.8 A Crystal structure of GDP-mannose dehydrogenase from P. aeruginosa PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Guanosine 5'-(Trihydrogen Diphosphate), P'-D-Mannopyranosyl Ester DB04023 DrugBank 5.1.4 P11759 GDP-mannose 6-dehydrogenase 100.0 436 7.2e-252
Sucrose DB02772 DrugBank 5.1.4 P11759 GDP-mannose 6-dehydrogenase 100.0 436 7.2e-252

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002126 (506 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3540
Search term: algD

References

Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa.
Franklin MJ, Ohman DE
J Bacteriol 2002 Jun;184(11):3000-7
PubMed ID: 12003941
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Pseudomonas aeruginosa infection in cystic fibrosis: nucleotide sequence and transcriptional regulation of the algD gene.
Deretic V, Gill JF, Chakrabarty AM
Nucleic Acids Res 1987 Jun 11;15(11):4567-81
PubMed ID: 3108855
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
Maharaj R, May TB, Wang SK, Chakrabarty AM
Gene 1993 Dec;136(1-2):267-9
PubMed ID: 8294014
GDP-mannose dehydrogenase is the key regulatory enzyme in alginate biosynthesis in Pseudomonas aeruginosa: evidence from metabolite studies.
Tatnell PJ, Russell NJ, Gacesa P
Microbiology (Reading, Engl.) 1994 Jul;140 ( Pt 7)
PubMed ID: 7521247