Pseudomonas aeruginosa PAO1, PA3544 (algE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3544
Name
algE
Synonym: alg76
Replicon chromosome
Genomic location 3968449 - 3969921 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_252234.1
GI 15598740
Affymetrix PA3544_algE_at
Entrez 879838
GenBank AAG06932.1
INSDC AAG06932.1
NCBI Locus Tag PA3544
protein_id(GenBank) gb|AAG06932.1|AE004775_3|gnl|PseudoCAP|PA3544
TIGR NTL03PA03544
UniParc UPI000012584E
UniProtKB Acc P18895
UniProtKB ID ALGE_PSEAE
UniRef100 UniRef100_P18895
UniRef50 UniRef50_P18895
UniRef90 UniRef90_P18895

Product

Feature Type CDS
Coding Frame 1
Product Name
Alginate production outer membrane protein AlgE precursor
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -20.33
Kyte-Doolittle Hydrophobicity Value -0.642
Molecular Weight (kDa) 54.4
Isoelectric Point (pI) 4.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4AFK MEMBRANE PROTEIN 01/19/12 In meso structure of alginate transporter, AlgE, from Pseudomonas aeruginosa, PAO1 PSEUDOMONAS AERUGINOSA 1.897 X-RAY DIFFRACTION 100.0
4B61 MEMBRANE PROTEIN 08/08/12 In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1. Crystal form 3. PSEUDOMONAS AERUGINOSA PAO1 2.402 X-RAY DIFFRACTION 100.0
5IYU MEMBRANE PROTEIN 03/24/16 AlgE_CIM PSEUDOMONAS AERUGINOSA PAO1 2.7 X-RAY DIFFRACTION 100.0
4AZL MEMBRANE PROTEIN 06/26/12 In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1, crystal form 2. PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 100.0
5D5D TRANSPORT PROTEIN 08/10/15 In meso in situ serial X-ray crystallography structure of AlgE at 100 K PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.4 X-RAY DIFFRACTION 100.0
4XNL TRANSPORT PROTEIN 01/15/15 X-ray structure of AlgE2 PSEUDOMONAS AERUGINOSA PAO1 2.9 X-RAY DIFFRACTION 100.0
3RBH TRANSPORT PROTEIN 03/29/11 Structure of alginate export protein AlgE from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.301 X-RAY DIFFRACTION 100.0
4XNK TRANSPORT PROTEIN 01/15/15 X-ray structure of AlgE1 PSEUDOMONAS AERUGINOSA PAO1 2.8 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002122 (512 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3544
Search term: algE

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 36041
Genomic Position 3969888
Position in ORF 1440(1473)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 46526
Genomic Position 3969887
Position in ORF 1439(1473)
Reference 14617778

Transposon Insertions in Orthologs

References

Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung.
Mathee K, Ciofu O, Sternberg C, Lindum PW, Campbell JI, Jensen P, Johnsen AH, Givskov M, Ohman DE, Molin S, Høiby N, Kharazmi A
Microbiology (Reading, Engl.) 1999 Jun;145 ( Pt 6)
PubMed ID: 10411261
Nucleotide sequence and expression of the algE gene involved in alginate biosynthesis by Pseudomonas aeruginosa.
Chu L, May TB, Chakrabarty AM, Misra TK
Gene 1991 Oct;107(1):1-10
PubMed ID: 1743507