Pseudomonas aeruginosa PAO1, PA3556 (arnT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3556
Name
arnT
Synonym: pqaB
Replicon chromosome
Genomic location 3984890 - 3986539 (+ strand)
Transposon Mutants 9 transposon mutants in PAO1

Cross-References

RefSeq NP_252246.1
GI 15598752
Affymetrix PA3556_at
Entrez 879122
GenBank AAG06944.1
INSDC AAG06944.1
NCBI Locus Tag PA3556
protein_id(GenBank) gb|AAG06944.1|AE004776_7|gnl|PseudoCAP|PA3556
TIGR NTL03PA03556
UniParc UPI00000C5A67
UniProtKB Acc Q9HY61
UniProtKB ID ARNT_PSEAE
UniRef100 UniRef100_Q9HY61
UniRef50 UniRef50_Q4K884
UniRef90 UniRef90_Q9HY61

Product

Feature Type CDS
Coding Frame 1
Product Name
inner membrane L-Ara4N transferase ArnT
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (61.73 kDa, PMID:21751344)
Charge (pH 7) 20.93
Kyte-Doolittle Hydrophobicity Value 0.399
Molecular Weight (kDa) 61.7
Isoelectric Point (pI) 10.10

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 243 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002110 (777 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3556
Search term: arnT

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_14_C11
Genomic Position 3986506
Position in ORF 1617(1650)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_39_E2
Genomic Position 3986506
Position in ORF 1617(1650)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 16684
Genomic Position 3985434
Position in ORF 545(1650)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_14_B1
Genomic Position 3986506
Position in ORF 1617(1650)
Reference 15805499
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 17287
Genomic Position 3985721
Position in ORF 832(1650)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_53_B9
Genomic Position 3986506
Position in ORF 1617(1650)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 43516
Genomic Position 3985812
Position in ORF 923(1650)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_68_H10
Genomic Position 3986506
Position in ORF 1617(1650)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_22_F5
Genomic Position 3986506
Position in ORF 1617(1650)
Reference 15805499

Transposon Insertions in Orthologs

References

An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor.
Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR
J. Biol. Chem. 2001 Nov;276(46):43122-31
PubMed ID: 11535604
PmrA-PmrB-regulated genes necessary for 4-aminoarabinose lipid A modification and polymyxin resistance.
Gunn JS, Lim KB, Krueger J, Kim K, Guo L, Hackett M, Miller SI
Mol. Microbiol. 1998 Mar;27(6):1171-82
PubMed ID: 9570402
The Salmonella typhi melittin resistance gene pqaB affects intracellular growth in PMA-differentiated U937 cells, polymyxin B resistance and lipopolysaccharide.
Baker SJ, Gunn JS, Morona R
Microbiology (Reading, Engl.) 1999 Feb;145 ( Pt 2)
PubMed ID: 10075419
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344