Pseudomonas aeruginosa PAO1, PA3561 (fruK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3561
Name
fruK
Replicon chromosome
Genomic location 3990597 - 3991541 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252251.1
GI 15598757
Affymetrix PA3561_fruK_at
DNASU PaCD00007561
Entrez 880224
GenBank AAG06949.1
INSDC AAG06949.1
NCBI Locus Tag PA3561
protein_id(GenBank) gb|AAG06949.1|AE004777_2|gnl|PseudoCAP|PA3561
TIGR NTL03PA03561
UniParc UPI00000C5A6B
UniProtKB Acc Q9HY56
UniProtKB ID Q9HY56_PSEAE
UniRef100 UniRef100_Q9HY56
UniRef50 UniRef50_Q9HY56
UniRef90 UniRef90_Q9HY56

Product

Feature Type CDS
Coding Frame 1
Product Name
1-phosphofructokinase
Product Name Confidence: Class 2
Synonyms fructose-1-phosphate kinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.05
Kyte-Doolittle Hydrophobicity Value 0.004
Molecular Weight (kDa) 33.4
Isoelectric Point (pI) 5.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 294 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002105 (521 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3561
Search term: fruK

Human Homologs

References

Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus.
Orchard LM, Kornberg HL
Proc Biol Sci 1990 Nov 22;242(1304):87-90
PubMed ID: 1981619