Pseudomonas aeruginosa PAO1, PA3622 (rpoS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3622
Name
rpoS
Replicon chromosome
Genomic location 4057909 - 4058913 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252312.1
GI 15598818
Affymetrix PA3622_rpoS_at
Entrez 880421
GenBank AAG07010.1
INSDC AAG07010.1
NCBI Locus Tag PA3622
protein_id(GenBank) gb|AAG07010.1|AE004782_8|gnl|PseudoCAP|PA3622
TIGR NTL03PA03622
UniParc UPI00001347E7
UniProtKB Acc P45684
UniProtKB ID RPOS_PSEAE
UniRef100 UniRef100_P45684
UniRef50 UniRef50_P45684
UniRef90 UniRef90_P45684

Product

Feature Type CDS
Coding Frame 1
Product Name
sigma factor RpoS
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.08
Kyte-Doolittle Hydrophobicity Value -0.547
Molecular Weight (kDa) 38.2
Isoelectric Point (pI) 5.05

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7XL4 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe) Pseudomonas aeruginosa PAO1 3.86 ELECTRON MICROSCOPY 100.0
7VF9 TRANSCRIPTION 09/10/21 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes Pseudomonas aeruginosa PAO1 4.04 ELECTRON MICROSCOPY 100.0
7XL3 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe) Pseudomonas aeruginosa PAO1 3.13 ELECTRON MICROSCOPY 100.0
7F0R TRANSCRIPTION/DNA 06/06/21 Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 5.8 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002052 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3622
Search term: rpoS
Search term: sigma factor RpoS

Human Homologs

References

Cloning, analysis and expression of an rpoS homologue gene from Pseudomonas aeruginosa PAO1.
Tanaka K, Takahashi H
Gene 1994 Dec 2;150(1):81-5
PubMed ID: 7959068
Effect of rpoS mutation on the stress response and expression of virulence factors in Pseudomonas aeruginosa.
Suh SJ, Silo-Suh L, Woods DE, Hassett DJ, West SE, Ohman DE
J Bacteriol 1999 Jul;181(13):3890-7
PubMed ID: 10383954