Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3636
|
Name |
kdsA
|
Replicon | chromosome |
Genomic location | 4070012 - 4070857 (- strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252326.1 |
GI | 15598832 |
Affymetrix | PA3636_kdsA_at |
DNASU | PaCD00007596 |
Entrez | 880428 |
GenBank | AAG07024.1 |
INSDC | AAG07024.1 |
NCBI Locus Tag | PA3636 |
protein_id(GenBank) | gb|AAG07024.1|AE004783_9|gnl|PseudoCAP|PA3636 |
TIGR | NTL03PA03636 |
UniParc | UPI000012DD5C |
UniProtKB Acc | Q9ZFK4 |
UniProtKB ID | KDSA_PSEAE |
UniRef100 | UniRef100_Q02RA8 |
UniRef50 | UniRef50_Q88LX0 |
UniRef90 | UniRef90_Q02RA8 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
2-dehydro-3-deoxyphosphooctonate aldolase
|
Synonyms |
3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase Kdo-8-P synthase |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -1.74 |
Kyte-Doolittle Hydrophobicity Value | -0.033 |
Molecular Weight (kDa) | 31.1 |
Isoelectric Point (pI) | 6.51 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4LU0 | TRANSFERASE | 07/24/13 | Crystal structure of 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa. | Pseudomonas aeruginosa | 2.8 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 504 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002038 (537 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3636
Search term: kdsA
Search term: 2-dehydro-3-deoxyphosphooctonate aldolase
|
The kdsA gene coding for 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase is part of an operon in Escherichia coli.
Woisetschläger M, Högenauer G
Mol Gen Genet 1987 May;207(2-3):369-73
PubMed ID: 3039295
|
Overproduction and one-step purification of Escherichia coli 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase and oxygen transfer studies during catalysis using isotopic-shifted heteronuclear NMR.
Dotson GD, Dua RK, Clemens JC, Wooten EW, Woodard RW
J Biol Chem 1995 Jun 9;270(23):13698-705
PubMed ID: 7775423
|
Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.
Strohmaier H, Remler P, Renner W, Högenauer G
J Bacteriol 1995 Aug;177(15):4488-500
PubMed ID: 7543480
|
Genetic and biochemical characterization of an operon involved in the biosynthesis of 3-deoxy-D-manno-octulosonic acid in Pseudomonas aeruginosa.
Walsh AG, Burrows LL, Lam JS
FEMS Microbiol Lett 1999 Apr 1;173(1):27-33
PubMed ID: 10220877
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|