Pseudomonas aeruginosa PAO1, PA3666 (dapD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3666
Name
dapD
Replicon chromosome
Genomic location 4104745 - 4105779 (+ strand)

Cross-References

RefSeq NP_252356.1
GI 15598862
Affymetrix PA3666_dapD_at
DNASU PaCD00007607
Entrez 880584
GenBank AAG07054.1
INSDC AAG07054.1
NCBI Locus Tag PA3666
protein_id(GenBank) gb|AAG07054.1|AE004786_6|gnl|PseudoCAP|PA3666
TIGR NTL03PA03666
UniParc UPI00000D427E
UniProtKB Acc G3XD76
UniProtKB ID DAPD_PSEAE
UniRef100 UniRef100_G3XD76
UniRef50 UniRef50_G3XD76
UniRef90 UniRef90_G3XD76

Product

Feature Type CDS
Coding Frame 1
Product Name
tetrahydrodipicolinate succinylase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.65
Kyte-Doolittle Hydrophobicity Value 0.208
Molecular Weight (kDa) 36.0
Isoelectric Point (pI) 5.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3R5A TRANSFERASE/TRANSFERASE INHIBITOR 03/18/11 Pseudomonas aeruginosa DapD (PA3666) in complex with D-2-aminopimelate Pseudomonas aeruginosa 1.89 X-RAY DIFFRACTION 100.0
3R5D TRANSFERASE 03/18/11 Pseudomonas aeruginosa DapD (PA3666) apoprotein Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 100.0
3R5C TRANSFERASE 03/18/11 Pseudomonas aeruginosa DapD (PA3666) in complex with CoA and succinate Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 100.0
3R5B TRANSFERASE/TRANSFERASE SUBSTRATE 03/18/11 Pseudomonas aeruginosa DapD (PA3666) in complex with L-2-aminopimelate Pseudomonas aeruginosa 2.51 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 95 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002008 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3666
Search term: dapD

Human Homologs

References

Different modes of diaminopimelate synthesis and their role in cell wall integrity: a study with Corynebacterium glutamicum.
Wehrmann A, Phillipp B, Sahm H, Eggeling L
J Bacteriol 1998 Jun;180(12):3159-65
PubMed ID: 9620966
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142