Pseudomonas aeruginosa PAO1, PA3735 (thrC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3735
Name
thrC
Replicon chromosome
Genomic location 4186781 - 4188190 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252424.1
GI 15598930
Affymetrix PA3735_thrC_at
Entrez 880336
GenBank AAG07122.1
INSDC AAG07122.1
NCBI Locus Tag PA3735
protein_id(GenBank) gb|AAG07122.1|AE004792_7|gnl|PseudoCAP|PA3735
TIGR NTL03PA03735
UniParc UPI0000136EDB
UniProtKB Acc P29363
UniProtKB ID THRC_PSEAE
UniRef100 UniRef100_P29363
UniRef50 UniRef50_P29363
UniRef90 UniRef90_P29363

Product

Feature Type CDS
Coding Frame 1
Product Name
threonine synthase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.32
Kyte-Doolittle Hydrophobicity Value -0.079
Molecular Weight (kDa) 51.8
Isoelectric Point (pI) 6.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4246
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 336 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001947 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3735
Search term: thrC

Human Homologs

Ensembl 110, assembly GRCh38.p14
threonine synthase like 2 [Source:HGNC Symbol;Acc:HGNC:25602]
E-value: 1.2e-79
Percent Identity: 35.9
Ensembl 110, assembly GRCh38.p14
threonine synthase like 2 [Source:HGNC Symbol;Acc:HGNC:25602]
E-value: 1.2e-79
Percent Identity: 35.9

References

Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes.
Clepet C, Borne F, Krishnapillai V, Baird C, Patte JC, Cami B
Mol Microbiol 1992 Nov;6(21):3109-19
PubMed ID: 1333566
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994