Pseudomonas aeruginosa PAO1, PA3761 (nagE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3761
Name
nagE
Synonym: nagE
Replicon chromosome
Genomic location 4212848 - 4214560 (+ strand)
Transposon Mutants 13 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252450.1
GI 15598956
Affymetrix PA3761_at
Entrez 880500
GenBank AAG07148.1
INSDC AAG07148.1
NCBI Locus Tag PA3761
protein_id(GenBank) gb|AAG07148.1|AE004794_13|gnl|PseudoCAP|PA3761
TIGR NTL03PA03761
UniParc UPI00000C5AFB
UniProtKB Acc Q9HXN4
UniProtKB ID Q9HXN4_PSEAE
UniRef100 UniRef100_Q9HXN4
UniRef50 UniRef50_A5I5S4
UniRef90 UniRef90_W0W922

Product

Feature Type CDS
Coding Frame 1
Product Name
N-Acetyl-D-Glucosamine phosphotransferase system transporter
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.54
Kyte-Doolittle Hydrophobicity Value 0.485
Molecular Weight (kDa) 60.6
Isoelectric Point (pI) 8.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 158 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001921 (384 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3761
Search term: nagE

Human Homologs

References

Comparison of the sequences of the nagE operons from Klebsiella pneumoniae and Escherichia coli K12: enhanced variability of the enzyme IIN-acetylglucosamine in regions connecting functional domains.
Vogler AP, Lengeler JW
Mol Gen Genet 1991 Nov;230(1-2):270-6
PubMed ID: 1745234
Pseudomonas aeruginosa enhances production of an antimicrobial in response to N-acetylglucosamine and peptidoglycan.
Korgaonkar AK, Whiteley M
J Bacteriol 2011 Feb;193(4):909-17
PubMed ID: 21169497