Pseudomonas aeruginosa PAO1, PA3764 (mltF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3764
Name
mltF
Synonym: yfhD
Replicon chromosome
Genomic location 4219841 - 4221199 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252453.1
GI 15598959
Affymetrix PA3764_at
Entrez 880530
GenBank AAG07151.1
INSDC AAG07151.1
NCBI Locus Tag PA3764
protein_id(GenBank) gb|AAG07151.1|AE004795_3|gnl|PseudoCAP|PA3764
TIGR NTL03PA03764
UniParc UPI00006D8743
UniProtKB Acc Q9HXN1
UniProtKB ID MLTF_PSEAE
UniRef100 UniRef100_Q9HXN1
UniRef50 UniRef50_Q9HXN1
UniRef90 UniRef90_Q9HXN1

Product

Feature Type CDS
Coding Frame 1
Product Name
lytic transglycosylase, MltF
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.06
Kyte-Doolittle Hydrophobicity Value -0.608
Molecular Weight (kDa) 51.1
Isoelectric Point (pI) 6.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5AA4 LYASE 07/23/15 Crystal structure of MltF from Pseudomonas aeruginosa in complex with cell-wall tetrapeptide PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 99.8
5AA2 LYASE 07/23/15 Crystal structure of MltF from Pseudomonas aeruginosa in complex with NAM-pentapeptide. PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 99.1
5AA1 LYASE 07/23/15 Crystal structure of MltF from Pseudomonas aeruginosa in complex with NAG-anhNAM-pentapeptide PSEUDOMONAS AERUGINOSA 2.89 X-RAY DIFFRACTION 99.3
5AA3 SUGAR BINDING PROTEIN 07/23/15 Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide PSEUDOMONAS AERUGINOSA 3.2 X-RAY DIFFRACTION 99.6
5A5X HYDROLASE 06/23/15 Crystal Structure of Se-Met MltF from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 1.801 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 244 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001917 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3764
Search term: mltF

Human Homologs

References

Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa.
Domínguez-Gil T, Lee M, Acebrón-Avalos I, Mahasenan KV, Hesek D, Dik DA, Byun B, Lastochkin E, Fisher JF, Mobashery S, Hermoso JA
Structure 2016 Oct 4;24(10):1729-1741
PubMed ID: 27618662