Pseudomonas aeruginosa PAO1, PA3800 (bamB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3800
Name	bamB
Replicon	chromosome
Genomic location	4259255 - 4260397 (- strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq	NP_252489.1
GI	15598995
Affymetrix	PA3800_at
Entrez	878230
GenBank	AAG07187.1
INSDC	AAG07187.1
NCBI Locus Tag	PA3800
protein_id(GenBank)	gb|AAG07187.1|AE004798_10|gnl|PseudoCAP|PA3800
TIGR	NTL03PA03800
UniParc	UPI00000C5B1B
UniProtKB Acc	Q9HXJ7
UniProtKB ID	BAMB_PSEAE
UniRef100	UniRef100_Q9HXJ7
UniRef50	UniRef50_Q9HXJ7
UniRef90	UniRef90_Q9HXJ7

Product

Feature Type	CDS
Coding Frame	1
Product Name	BamB, component of beta-Barrel Assembly Machine Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Detected using LC-MS/MS (40.40 kDa, PMID:21751344) Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-4.54
Kyte-Doolittle Hydrophobicity Value	0.024
Molecular Weight (kDa)	40.4
Isoelectric Point (pI)	5.11

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Class 3 20472543 Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4HDJ	PROTEIN BINDING	10/02/12	Crystal Structure of BamB from Pseudomonas aeruginosa	Pseudomonas aeruginosa	1.85	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 268 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001866 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3800 Search term: bamB Search term: BamB, component of beta-Barrel Assembly Machine

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix. Toyofuku M, Roschitzki B, Riedel K, Eberl L J Proteome Res 2012 Oct 5;11(10):4906-15 PubMed ID: 22909304

Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms. Couto N, Schooling SR, Dutcher JR, Barber J J Proteome Res 2015 Oct 2;14(10):4207-22 PubMed ID: 26303878

Crystal structure of BamB from Pseudomonas aeruginosa and functional evaluation of its conserved structural features. Jansen KB, Baker SL, Sousa MC PLoS One 2012;7(11):e49749 PubMed ID: 23189157