Pseudomonas aeruginosa PAO1, PA3807 (ndk)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3807
Name
ndk
Replicon chromosome
Genomic location 4266470 - 4266901 (- strand)
CommentSecreted by mucoid strain Pseudomonas aeruginosa 8821

Cross-References

RefSeq NP_252496.1
GI 15599002
Affymetrix PA3807_ndk_at
Entrez 879892
GenBank AAG07194.1
INSDC AAG07194.1
NCBI Locus Tag PA3807
protein_id(GenBank) gb|AAG07194.1|AE004798_17|gnl|PseudoCAP|PA3807
TIGR NTL03PA03807
UniParc UPI000012FEBA
UniProtKB Acc Q59636
UniProtKB ID NDK_PSEAE
UniRef100 UniRef100_A6V0V6
UniRef50 UniRef50_Q5WGT0
UniRef90 UniRef90_A6V0V6

Product

Feature Type CDS
Coding Frame 1
Product Name
nucleoside diphosphate kinase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -2.55
Kyte-Doolittle Hydrophobicity Value -0.089
Molecular Weight (kDa) 15.6
Isoelectric Point (pI) 5.31

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 598 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001859 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3807
Search term: ndk

Human Homologs

Ensembl 94, assembly GRCh38.p12
NME/NM23 nucleoside diphosphate kinase 3 [Source:HGNC Symbol;Acc:HGNC:7851]
E-value: 5.9e-31
Percent Identity: 48.9

References

Nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of the gene and its role in cellular growth and exopolysaccharide alginate synthesis.
Sundin GW, Shankar S, Chugani SA, Chopade BA, Kavanaugh-Black A, Chakrabarty AM
Mol. Microbiol. 1996 Jun;20(5):965-79
PubMed ID: 8809750
Secretion of nucleoside diphosphate kinase by mucoid Pseudomonas aeruginosa 8821: involvement of a carboxy-terminal motif in secretion.
Kamath S, Chen ML, Chakrabarty AM
J. Bacteriol. 2000 Jul;182(13):3826-31
PubMed ID: 10851000
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142