Pseudomonas aeruginosa PAO1, PA3807 (ndk)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA3807
|
| Name |
ndk
|
| Replicon | chromosome |
| Genomic location | 4266470 - 4266901 (- strand) |
| Comment | Secreted by mucoid strain Pseudomonas aeruginosa 8821 |
Cross-References
| RefSeq | NP_252496.1 |
| GI | 15599002 |
| Affymetrix | PA3807_ndk_at |
| Entrez | 879892 |
| GenBank | AAG07194.1 |
| INSDC | AAG07194.1 |
| NCBI Locus Tag | PA3807 |
| protein_id(GenBank) | gb|AAG07194.1|AE004798_17|gnl|PseudoCAP|PA3807 |
| TIGR | NTL03PA03807 |
| UniParc | UPI000012FEBA |
| UniProtKB Acc | Q59636 |
| UniProtKB ID | NDK_PSEAE |
| UniRef100 | UniRef100_A6V0V6 |
| UniRef50 | UniRef50_Q5WGT0 |
| UniRef90 | UniRef90_A6V0V6 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
nucleoside diphosphate kinase
|
| Synonyms | |
| Evidence for Translation |
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -2.55 |
| Kyte-Doolittle Hydrophobicity Value | -0.089 |
| Molecular Weight (kDa) | 15.6 |
| Isoelectric Point (pI) | 5.31 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 6AES | TRANSFERASE | 08/06/18 | Crystal structure of Nucleoside diphosphate kinase from Pseudomonas aeruginosa at 3.55 A resolution. | Pseudomonas aeruginosa | 3.55 | X-RAY DIFFRACTION | 100.0 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 598 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG001859 (536 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA3807
Search term: ndk
Search term: nucleoside diphosphate kinase
|
Human Homologs
|
Ensembl
110, assembly
GRCh38.p14
|
NME/NM23 family member 7 [Source:HGNC Symbol;Acc:HGNC:20461]
E-value:
2.0e-22
Percent Identity:
41.7
|
|
Ensembl
110, assembly
GRCh38.p14
|
NME/NM23 family member 7 [Source:HGNC Symbol;Acc:HGNC:20461]
E-value:
2.0e-22
Percent Identity:
41.7
|
|
Ensembl
110, assembly
GRCh38.p14
|
NME/NM23 family member 7 [Source:HGNC Symbol;Acc:HGNC:20461]
E-value:
2.0e-22
Percent Identity:
41.7
|
|
Ensembl
110, assembly
GRCh38.p14
|
NME/NM23 family member 7 [Source:HGNC Symbol;Acc:HGNC:20461]
E-value:
2.0e-22
Percent Identity:
41.7
|
References
|
Nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of the gene and its role in cellular growth and exopolysaccharide alginate synthesis.
Sundin GW, Shankar S, Chugani SA, Chopade BA, Kavanaugh-Black A, Chakrabarty AM
Mol Microbiol 1996 Jun;20(5):965-79
PubMed ID: 8809750
|
|
Secretion of nucleoside diphosphate kinase by mucoid Pseudomonas aeruginosa 8821: involvement of a carboxy-terminal motif in secretion.
Kamath S, Chen ML, Chakrabarty AM
J Bacteriol 2000 Jul;182(13):3826-31
PubMed ID: 10851000
|
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|