Pseudomonas aeruginosa PAO1, PA3814 (iscS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3814
Name
iscS
Replicon chromosome
Genomic location 4270921 - 4272135 (- strand)
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_252503.1
GI 15599009
Affymetrix PA3814_iscS_at
Entrez 879918
GenBank AAG07201.1
INSDC AAG07201.1
NCBI Locus Tag PA3814
protein_id(GenBank) gb|AAG07201.1|AE004799_7|gnl|PseudoCAP|PA3814
TIGR NTL03PA03814
UniParc UPI000012D8DC
UniProtKB Acc Q9HXI8
UniProtKB ID ISCS_PSEAE
UniRef100 UniRef100_Q02RW8
UniRef50 UniRef50_Q486Z0
UniRef90 UniRef90_A6V0U8

Product

Feature Type CDS
Coding Frame 1
Product Name
L-cysteine desulfurase (pyridoxal phosphate-dependent)
Product Name Confidence: Class 2
Synonyms IscS protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.94
Kyte-Doolittle Hydrophobicity Value -0.253
Molecular Weight (kDa) 44.7
Isoelectric Point (pI) 5.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 652 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001852 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3814
Search term: iscS

Human Homologs

Ensembl 110, assembly GRCh38.p14
NFS1 cysteine desulfurase [Source:HGNC Symbol;Acc:HGNC:15910]
E-value: 5.7e-131
Percent Identity: 56.2

References

Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii.
Zheng L, Cash VL, Flint DH, Dean DR
J Biol Chem 1998 May 22;273(21):13264-72
PubMed ID: 9582371