Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3831
|
Name |
pepA
Synonym: xerB Synonym: phpA Synonym: carP |
Replicon | chromosome |
Genomic location | 4288943 - 4290430 (+ strand) |
Transposon Mutants | 7 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252520.1 |
GI | 15599026 |
Affymetrix | PA3831_pepA_at |
Entrez | 879865 |
GenBank | AAG07218.1 |
INSDC | AAG07218.1 |
NCBI Locus Tag | PA3831 |
protein_id(GenBank) | gb|AAG07218.1|AE004800_7|gnl|PseudoCAP|PA3831 |
TIGR | NTL03PA03831 |
UniParc | UPI00001259C4 |
UniProtKB Acc | O68822 |
UniProtKB ID | AMPA_PSEAE |
UniRef100 | UniRef100_O68822 |
UniRef50 | UniRef50_P68768 |
UniRef90 | UniRef90_O68822 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
leucine aminopeptidase
|
Synonyms |
Aminopeptidase A |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (52.33 kDa, PMID:21751344)
|
Charge (pH 7) | 6.04 |
Kyte-Doolittle Hydrophobicity Value | -0.007 |
Molecular Weight (kDa) | 52.3 |
Isoelectric Point (pI) | 8.74 |
Individual Mappings | |
Additional evidence for subcellular localization |
Source 1 | |
Database | PseudoCAP |
Category | PepA |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Mus musculus |
Notes | pepA mutant is necessary for virulence in mouse model of acute pneumonia |
Infection model | mouse model of acute pneumonia |
Reference | 9515706 |
Source 2 | |
Database | Victors : 4238 |
Category | PepA |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Rattus norvegicus |
Notes | |
Infection model | Rat agar bead model of chronic lung infection |
Reference | 14641575 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 453 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001835 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3831
Search term: pepA
Search term: leucine aminopeptidase
|
Ensembl
110, assembly
GRCh38.p14
|
leucine aminopeptidase 3 [Source:HGNC Symbol;Acc:HGNC:18449]
E-value:
9.0e-70
Percent Identity:
38.3
|
Ensembl
110, assembly
GRCh38.p14
|
leucine aminopeptidase 3 [Source:HGNC Symbol;Acc:HGNC:18449]
E-value:
9.0e-70
Percent Identity:
38.3
|
Ensembl
110, assembly
GRCh38.p14
|
leucine aminopeptidase 3 [Source:HGNC Symbol;Acc:HGNC:18449]
E-value:
9.0e-70
Percent Identity:
38.3
|
xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase.
Stirling CJ, Colloms SD, Collins JF, Szatmari G, Sherratt DJ
EMBO J 1989 May;8(5):1623-7
PubMed ID: 2670557
|
Peptidase activity of Escherichia coli aminopeptidase A is not required for its role in Xer site-specific recombination.
McCulloch R, Burke ME, Sherratt DJ
Mol Microbiol 1994 Apr;12(2):241-51
PubMed ID: 8057849
|
carP, involved in pyrimidine regulation of the Escherichia coli carbamoylphosphate synthetase operon encodes a sequence-specific DNA-binding protein identical to XerB and PepA, also required for resolution of ColEI multimers.
Charlier D, Hassanzadeh G, Kholti A, Gigot D, Piérard A, Glansdorff N
J Mol Biol 1995 Jul 21;250(4):392-406
PubMed ID: 7616564
|
Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa.
Woolwine SC, Wozniak DJ
J Bacteriol 1999 Jan;181(1):107-16
PubMed ID: 9864319
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
PepA, a secreted protein of Pseudomonas aeruginosa, is necessary for cytotoxicity and virulence.
Hauser AR, Kang PJ, Engel JN
Mol Microbiol 1998 Feb;27(4):807-18
PubMed ID: 9515706
|