Pseudomonas aeruginosa PAO1, PA3831 (pepA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3831
Name
pepA
Synonym: xerB
Synonym: phpA
Synonym: carP
Replicon chromosome
Genomic location 4288943 - 4290430 (+ strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252520.1
GI 15599026
Affymetrix PA3831_pepA_at
Entrez 879865
GenBank AAG07218.1
INSDC AAG07218.1
NCBI Locus Tag PA3831
protein_id(GenBank) gb|AAG07218.1|AE004800_7|gnl|PseudoCAP|PA3831
TIGR NTL03PA03831
UniParc UPI00001259C4
UniProtKB Acc O68822
UniProtKB ID AMPA_PSEAE
UniRef100 UniRef100_O68822
UniRef50 UniRef50_P68768
UniRef90 UniRef90_O68822

Product

Feature Type CDS
Coding Frame 1
Product Name
leucine aminopeptidase
Product Name Confidence: Class 1
Synonyms Aminopeptidase A
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (52.33 kDa, PMID:21751344)
Charge (pH 7) 6.04
Kyte-Doolittle Hydrophobicity Value -0.007
Molecular Weight (kDa) 52.3
Isoelectric Point (pI) 8.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Extracellular Class 1
Western blot evidence used in manual assertion
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category PepA
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes pepA mutant is necessary for virulence in mouse model of acute pneumonia
Infection model mouse model of acute pneumonia
Reference 9515706
Source 2
Database Victors : 4238
Category PepA
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 453 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001835 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3831
Search term: pepA

Human Homologs

Ensembl 110, assembly GRCh38.p14
leucine aminopeptidase 3 [Source:HGNC Symbol;Acc:HGNC:18449]
E-value: 9.0e-70
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
leucine aminopeptidase 3 [Source:HGNC Symbol;Acc:HGNC:18449]
E-value: 9.0e-70
Percent Identity: 38.3
Ensembl 110, assembly GRCh38.p14
leucine aminopeptidase 3 [Source:HGNC Symbol;Acc:HGNC:18449]
E-value: 9.0e-70
Percent Identity: 38.3

References

xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase.
Stirling CJ, Colloms SD, Collins JF, Szatmari G, Sherratt DJ
EMBO J 1989 May;8(5):1623-7
PubMed ID: 2670557
Peptidase activity of Escherichia coli aminopeptidase A is not required for its role in Xer site-specific recombination.
McCulloch R, Burke ME, Sherratt DJ
Mol Microbiol 1994 Apr;12(2):241-51
PubMed ID: 8057849
carP, involved in pyrimidine regulation of the Escherichia coli carbamoylphosphate synthetase operon encodes a sequence-specific DNA-binding protein identical to XerB and PepA, also required for resolution of ColEI multimers.
Charlier D, Hassanzadeh G, Kholti A, Gigot D, Piérard A, Glansdorff N
J Mol Biol 1995 Jul 21;250(4):392-406
PubMed ID: 7616564
Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa.
Woolwine SC, Wozniak DJ
J Bacteriol 1999 Jan;181(1):107-16
PubMed ID: 9864319
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
PepA, a secreted protein of Pseudomonas aeruginosa, is necessary for cytotoxicity and virulence.
Hauser AR, Kang PJ, Engel JN
Mol Microbiol 1998 Feb;27(4):807-18
PubMed ID: 9515706