Pseudomonas aeruginosa PAO1, PA3832 (holC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3832
Name
holC
Replicon chromosome
Genomic location 4290427 - 4290855 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252521.1
GI 15599027
Affymetrix PA3832_holC_at
DNASU PaCD00007670
Entrez 879882
GenBank AAG07219.1
INSDC AAG07219.1
NCBI Locus Tag PA3832
protein_id(GenBank) gb|AAG07219.1|AE004800_8|gnl|PseudoCAP|PA3832
TIGR NTL03PA03832
UniParc UPI000012CACC
UniProtKB Acc O68823
UniProtKB ID HOLC_PSEAE
UniRef100 UniRef100_O68823
UniRef50 UniRef50_O68823
UniRef90 UniRef90_O68823

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase III, chi subunit
Product Name Confidence: Class 2
Synonyms HolC
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.11
Kyte-Doolittle Hydrophobicity Value -0.366
Molecular Weight (kDa) 16.1
Isoelectric Point (pI) 5.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 141 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001834 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3832
Search term: holC

Human Homologs

References

DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi.
Xiao H, Crombie R, Dong Z, Onrust R, O'Donnell M
J Biol Chem 1993 Jun 5;268(16):11773-8
PubMed ID: 8389364
DNA polymerase III accessory proteins. IV. Characterization of chi and psi.
Xiao H, Dong Z, O'Donnell M
J Biol Chem 1993 Jun 5;268(16):11779-84
PubMed ID: 8505305
Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli.
Carter JR, Franden MA, Lippincott JA, McHenry CS
Mol Gen Genet 1993 Nov;241(3-4):399-408
PubMed ID: 8246893