Pseudomonas aeruginosa PAO1, PA3842 (spcS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3842
Name
spcS
Synonym: orf1
Replicon chromosome
Genomic location 4304690 - 4305040 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252531.1
GI 15599037
Affymetrix PA3842_at
Entrez 879848
GenBank AAG07229.1
INSDC AAG07229.1
NCBI Locus Tag PA3842
protein_id(GenBank) gb|AAG07229.1|AE004801_7|gnl|PseudoCAP|PA3842
TIGR NTL03PA03842
UniParc UPI00000D427A
UniProtKB Acc G3XD93
UniProtKB ID G3XD93_PSEAE
UniRef100 UniRef100_Q51450
UniRef50 UniRef50_A4STG9
UniRef90 UniRef90_Q51450

Product

Feature Type CDS
Coding Frame 1
Product Name
specific Pseudomonas chaperone for ExoS, SpcS
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -7.39
Kyte-Doolittle Hydrophobicity Value -0.427
Molecular Weight (kDa) 13.2
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6JNP TOXIN 03/17/19 Structure of ExoT-SpcS Complex from Pseudomonas aeruginosa in 2.2 Angstrom Pseudomonas aeruginosa 2.261 X-RAY DIFFRACTION 100.0
4JMF TOXIN/CHAPERONE 03/14/13 Crystal structure of ExoT (residues 28 -77)- SpcS complex from Pseudomonas aeruginosa at 2.1 angstrom Pseudomonas aeruginosa 2.099 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 3 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001825 (301 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3842
Search term: spcS

Human Homologs

References

SycE, a chaperone-like protein of Yersinia enterocolitica involved in Ohe secretion of YopE.
Wattiau P, Cornelis GR
Mol Microbiol 1993 Apr;8(1):123-31
PubMed ID: 8497188
Transcriptional analysis of the Pseudomonas aeruginosa exoenzyme S structural gene.
Yahr TL, Hovey AK, Kulich SM, Frank DW
J Bacteriol 1995 Mar;177(5):1169-78
PubMed ID: 7868588
Intracellular targeting of exoenzyme S of Pseudomonas aeruginosa via type III-dependent translocation induces phagocytosis resistance, cytotoxicity and disruption of actin microfilaments.
Frithz-Lindsten E, Du Y, Rosqvist R, Forsberg A
Mol Microbiol 1997 Sep;25(6):1125-39
PubMed ID: 9350868
Orf1/SpcS chaperones ExoS for type three secretion by Pseudomonas aeruginosa.
Shen DK, Quenee L, Bonnet M, Kuhn L, Derouazi M, Lamotte D, Toussaint B, Polack B
Biomed Environ Sci 2008 Apr;21(2):103-9
PubMed ID: 18548848