Pseudomonas aeruginosa PAO1, PA3976 (thiE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3976
Name
thiE
Synonym: thiC
Replicon chromosome
Genomic location 4456695 - 4457324 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252665.1
GI 15599171
Affymetrix PA3976_thiE_at
DNASU PaCD00007737
Entrez 880858
GenBank AAG07363.1
INSDC AAG07363.1
NCBI Locus Tag PA3976
protein_id(GenBank) gb|AAG07363.1|AE004815_7|gnl|PseudoCAP|PA3976
TIGR NTL03PA03976
UniParc UPI00000C5BAC
UniProtKB Acc Q9HX40
UniProtKB ID THIE_PSEAE
UniRef100 UniRef100_Q9HX40
UniRef50 UniRef50_Q9HX40
UniRef90 UniRef90_Q9HX40

Product

Feature Type CDS
Coding Frame 1
Product Name
thiamin-phosphate pyrophosphorylase
Product Name Confidence: Class 2
Synonyms thiamin-phosphate synthase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.14
Kyte-Doolittle Hydrophobicity Value 0.076
Molecular Weight (kDa) 22.1
Isoelectric Point (pI) 7.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 542 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001705 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3976
Search term: thiE

Human Homologs

References

Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.
Vander Horn PB, Backstrom AD, Stewart V, Begley TP
J Bacteriol 1993 Feb;175(4):982-92
PubMed ID: 8432721
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
Zhang Y, Taylor SV, Chiu HJ, Begley TP
J Bacteriol 1997 May;179(9):3030-5
PubMed ID: 9139923