Pseudomonas aeruginosa PAO1, PA3989 (holA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3989
Name
holA
Replicon chromosome
Genomic location 4470274 - 4471311 (+ strand)

Cross-References

RefSeq NP_252678.1
GI 15599184
Affymetrix PA3989_holA_at
Entrez 878936
GenBank AAG07376.1
INSDC AAG07376.1
NCBI Locus Tag PA3989
protein_id(GenBank) gb|AAG07376.1|AE004816_12|gnl|PseudoCAP|PA3989
TIGR NTL03PA03989
UniParc UPI00000C5BB3
UniProtKB Acc Q9HX31
UniProtKB ID Q9HX31_PSEAE
UniRef100 UniRef100_Q9HX31
UniRef50 UniRef50_M4XB34
UniRef90 UniRef90_A6V0C0

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase III, delta subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.12
Kyte-Doolittle Hydrophobicity Value -0.074
Molecular Weight (kDa) 37.4
Isoelectric Point (pI) 6.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 250 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001692 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3989
Search term: holA

Human Homologs

References

Molecular cloning, sequencing, and overexpression of the structural gene encoding the delta subunit of Escherichia coli DNA polymerase III holoenzyme.
Carter JR, Franden MA, Aebersold R, McHenry CS
J Bacteriol 1992 Nov;174(21):7013-25
PubMed ID: 1400251
DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'.
Dong Z, Onrust R, Skangalis M, O'Donnell M
J Biol Chem 1993 Jun 5;268(16):11758-65
PubMed ID: 8505303
DNA polymerase III accessory proteins. II. Characterization of delta and delta'.
Onrust R, O'Donnell M
J Biol Chem 1993 Jun 5;268(16):11766-72
PubMed ID: 8505304