Pseudomonas aeruginosa PAO1, PA4003 (pbpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4003
Name
pbpA
Synonym: mrdA
Replicon chromosome
Genomic location 4483396 - 4485336 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252692.1
GI 15599198
Affymetrix PA4003_pbpA_at
Entrez 878962
GenBank AAG07390.1
INSDC AAG07390.1
NCBI Locus Tag PA4003
protein_id(GenBank) gb|AAG07390.1|AE004817_14|gnl|PseudoCAP|PA4003
TIGR NTL03PA04003
UniParc UPI00000D4222
UniProtKB Acc G3XCV7
UniProtKB ID G3XCV7_PSEAE
UniRef100 UniRef100_G3XCV7
UniRef50 UniRef50_G8PXZ8
UniRef90 UniRef90_G3XCV7

Product

Feature Type CDS
Coding Frame 1
Product Name
penicillin-binding protein 2
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (72.21 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 5.83
Kyte-Doolittle Hydrophobicity Value -0.332
Molecular Weight (kDa) 72.2
Isoelectric Point (pI) 8.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7KIS HYDROLASE/Inhibitor/Antibiotic 10/24/20 Crystal structure of Pseudomonas aeruginosa PBP2 in complex with WCK 5153 Pseudomonas aeruginosa 2.869 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Ceftazidime DB00438 DrugBank 5.1.4 Q9X6V3 Penicillin-binding protein 2 100.0 646 0.0e+00
Cefepime DB01413 DrugBank 5.1.4 Q9X6V3 Penicillin-binding protein 2 100.0 646 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 575 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001680 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4003
Search term: pbpA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli.
Tamaki S, Matsuzawa H, Matsuhashi M
J Bacteriol 1980 Jan;141(1):52-7
PubMed ID: 6243629
Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12.
Asoh S, Matsuzawa H, Ishino F, Strominger JL, Matsuhashi M, Ohta T
Eur J Biochem 1986 Oct 15;160(2):231-8
PubMed ID: 3533535
Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon.
Matsuzawa H, Asoh S, Kunai K, Muraiso K, Takasuga A, Ohta T
J Bacteriol 1989 Jan;171(1):558-60
PubMed ID: 2644207
Penicillin-binding protein 2 inactivation in Escherichia coli results in cell division inhibition, which is relieved by FtsZ overexpression.
Vinella D, Joseleau-Petit D, Thévenet D, Bouloc P, D'Ari R
J Bacteriol 1993 Oct;175(20):6704-10
PubMed ID: 8407846
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344