Pseudomonas aeruginosa PAO1, PA4031 (ppa)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4031
Name
ppa
Synonym: ipyR
Replicon chromosome
Genomic location 4512549 - 4513076 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252720.1
GI 15599226
Affymetrix PA4031_ppa_at
Entrez 879025
GenBank AAG07418.1
INSDC AAG07418.1
NCBI Locus Tag PA4031
protein_id(GenBank) gb|AAG07418.1|AE004820_3|gnl|PseudoCAP|PA4031
TIGR NTL03PA04031
UniParc UPI000012D84B
UniProtKB Acc Q9HWZ6
UniProtKB ID IPYR_PSEAE
UniRef100 UniRef100_Q9HWZ6
UniRef50 UniRef50_Q9HWZ6
UniRef90 UniRef90_Q9HWZ6

Product

Feature Type CDS
Coding Frame 1
Product Name
inorganic pyrophosphatase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) -6.08
Kyte-Doolittle Hydrophobicity Value -0.142
Molecular Weight (kDa) 19.4
Isoelectric Point (pI) 4.86

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4XEL HYDROLASE 12/24/14 Crystal structure of Inorganic pyrophosphatase (PPase) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
5-amino-1-(4-chlorophenyl)-1H-pyrazole-4-carbonitrile DB07291 DrugBank 5.1.4 Q3JUV5 Inorganic pyrophosphatase 62.9 175 3.4e-59
N-(pyridin-3-ylmethyl)aniline DB06851 DrugBank 5.1.4 Q3JUV5 Inorganic pyrophosphatase 62.9 175 3.4e-59

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 482 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001653 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4031
Search term: ppa

Human Homologs

References

Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12.
Lahti R, Pitkäranta T, Valve E, Ilta I, Kukko-Kalske E, Heinonen J
J Bacteriol 1988 Dec;170(12):5901-7
PubMed ID: 2848015
The structure of E.coli soluble inorganic pyrophosphatase at 2.7 A resolution.
Kankare J, Neal GS, Salminen T, Glumoff T, Glumhoff T [corrected to Glumoff T], Cooperman BS, Lahti R, Goldman A
Protein Eng 1994 Jul;7(7):823-30
PubMed ID: 7971944
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299