Pseudomonas aeruginosa PAO1, PA4053 (ribE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4053
Name
ribE
Synonym: ribH
Replicon chromosome
Genomic location 4534116 - 4534592 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252742.1
GI 15599248
Affymetrix PA4053_ribE_at
DNASU PaCD00007772
Entrez 879081
GenBank AAG07440.1
INSDC AAG07440.1
NCBI Locus Tag PA4053
protein_id(GenBank) gb|AAG07440.1|AE004821_13|gnl|PseudoCAP|PA4053
TIGR NTL03PA04053
UniParc UPI00001339BF
UniProtKB Acc Q9HWX5
UniProtKB ID RISB_PSEAE
UniRef100 UniRef100_A6V049
UniRef50 UniRef50_P45149
UniRef90 UniRef90_Q889Q6

Product

Feature Type CDS
Coding Frame 1
Product Name
6,7-dimethyl-8-ribityllumazine synthase
Product Name Confidence: Class 2
Synonyms riboflavin synthase beta chain
lumazine synthase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.56
Kyte-Doolittle Hydrophobicity Value 0.449
Molecular Weight (kDa) 16.4
Isoelectric Point (pI) 5.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 612 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001631 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4053
Search term: ribE

Human Homologs

References

Heavy riboflavin synthase of Bacillus subtilis. Primary structure of the beta subunit.
Ludwig HC, Lottspeich F, Henschen A, Ladenstein R, Bacher A
J Biol Chem 1987 Jan 25;262(3):1016-21
PubMed ID: 3100522
Riboflavin synthesis genes are linked with the lux operon of Photobacterium phosphoreum.
Lee CY, O'Kane DJ, Meighen EA
J Bacteriol 1994 Apr;176(7):2100-4
PubMed ID: 8144477
Crystallization and preliminary x-ray diffraction analysis of the lumazine synthase from Brucella abortus.
Goldbaum FA, Polikarpov I, Cauerhff AA, Velikovsky CA, Braden BC, Poljak RJ
J Struct Biol 1998 Oct;123(2):175-8
PubMed ID: 9843672