Pseudomonas aeruginosa PAO1, PA4110 (ampC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4110
Name
ampC
Replicon chromosome
Genomic location 4594029 - 4595222 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252799.1
GI 15599305
Affymetrix PA4110_ampC_at
Entrez 878149
GenBank AAG07497.1
INSDC ACQ82807.1
NCBI Locus Tag PA4110
protein_id(GenBank) gb|AAG07497.1|AE004827_6|gnl|PseudoCAP|PA4110
TIGR NTL03PA04110
UniParc UPI0000001142
UniProtKB Acc Q541D8
UniProtKB Acc P24735
UniProtKB ID Q541D8_PSEAE
UniProtKB ID Q541D8_PSEAI
UniRef100 UniRef100_P24735
UniRef50 UniRef50_P85302
UniRef90 UniRef90_P24735

Product

Feature Type CDS
Coding Frame 1
Product Name
beta-lactamase precursor
Product Name Confidence: Class 1
Synonyms cephalosporinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.38
Kyte-Doolittle Hydrophobicity Value -0.290
Molecular Weight (kDa) 43.4
Isoelectric Point (pI) 8.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
PDC-1 PDC-1 is a beta-lactamase found in Pseudomonas aeruginosa. [PMID:19258272] ARO:3002497 protein homolog model Perfect 807.4 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search
Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8SDT HYDROLASE 04/07/23 Crystal structure of PDC-3 beta-lactamase in complex with the boronic acid inhibitor S02030 Pseudomonas aeruginosa 1.38 X-RAY DIFFRACTION 99.7
4WYY hydrolase/hydrolase inhibitor 11/18/14 Crystal Structure of P. aeruginosa AmpC Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 1.28 X-RAY DIFFRACTION 100.0
6UQU ANTIBIOTIC 10/21/19 Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors Pseudomonas aeruginosa 1.0902 X-RAY DIFFRACTION 100.0
8SDN HYDROLASE 04/07/23 Crystal structure of PDC-3 Y221H beta-lactamase Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 99.5
6UQT ANTIBIOTIC 10/21/19 Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors Pseudomonas aeruginosa 1.25 X-RAY DIFFRACTION 100.0
6UR3 ANTIBIOTIC 10/22/19 Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors Pseudomonas aeruginosa 1.423 X-RAY DIFFRACTION 100.0
3S22 Hydrolase/Hydrolase Inhibitor 05/16/11 AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA) in complex with an inhibitor Pseudomonas aeruginosa 1.65 X-RAY DIFFRACTION 100.0
6I30 HYDROLASE 11/02/18 Crystal structure of the AmpC from Pseudomonas aeruginosa with 1C Pseudomonas aeruginosa 2.21 X-RAY DIFFRACTION 100.0
8SDS HYDROLASE 04/07/23 Crystal structure of PDC-3 Y221H beta-lactamase in complex with the boronic acid inhibitor LP-06 Pseudomonas aeruginosa 1.63 X-RAY DIFFRACTION 99.5
6S1S HYDROLASE 06/19/19 Crystal structure of AmpC from Pseudomonas aeruginosa in complex with [3-(2-carboxyvinyl)phenyl]boronic acid] inhibitor Pseudomonas aeruginosa 1.78 X-RAY DIFFRACTION 100.0
8SDV HYDROLASE 04/07/23 Crystal structure of PDC-3 Y221H beta-lactamase in complex with the boronic acid inhibitor S02030 Pseudomonas aeruginosa 1.42 X-RAY DIFFRACTION 99.5
4WZ4 hydrolase/hydrolase inhibitor 11/18/14 Crystal structure of P. aeruginosa AmpC Pseudomonas aeruginosa PAO1 1.05 X-RAY DIFFRACTION 100.0
6UQS ANTIBIOTIC 10/21/19 Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors Pseudomonas aeruginosa 1.37 X-RAY DIFFRACTION 100.0
4OOY hydrolase/hydrolase inhibitor 02/04/14 Avibactam and class C beta-lactamases: mechanism of inhibition, conservation of binding pocket and implications for resistance Pseudomonas aeruginosa 1.1 X-RAY DIFFRACTION 100.0
2WZZ HYDROLASE 12/03/09 AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA)IN COMPLEX WITH compound M-03 PSEUDOMONAS AERUGINOSA 1.57 X-RAY DIFFRACTION 100.0
4NK3 HYDROLASE/HYDROLASE INHIBITOR 11/12/13 Amp-c beta-lactamase (pseudomonas aeruginosa) in complex with mk-7655 Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
4HEF HYDROLASE/HYDROLASE INHIBITOR 10/03/12 Structure of avibactam bound to Pseudomonas aeruginosa AmpC Pseudomonas aeruginosa 1.86 X-RAY DIFFRACTION 99.7
4GZB HYDROLASE 09/06/12 Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa 1.79 X-RAY DIFFRACTION 100.0
2WZX HYDROLASE 12/03/09 AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA)IN COMPLEX WITH compound M-02 PSEUDOMONAS AERUGINOSA 1.4 X-RAY DIFFRACTION 100.0
8SDL HYDROLASE 04/07/23 Crystal structure of PDC-3 beta-lactamase Pseudomonas aeruginosa 1.75 X-RAY DIFFRACTION 99.7
8SDR HYDROLASE/HYDROLASE INHIBITOR 04/07/23 Crystal structure of PDC-3 beta-lactamase in complex with the boronic acid inhibitor LP-06 Pseudomonas aeruginosa 1.35 X-RAY DIFFRACTION 99.7
4X68 HYDROLASE/HYDROLASE INHIBITOR 12/07/14 Crystal Structure of OP0595 complexed with AmpC Pseudomonas aeruginosa 1.68 X-RAY DIFFRACTION 100.0
3S1Y Hydrolase/Hydrolase Inhibitor 05/16/11 AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA) in complex with a beta-lactamase inhibitor Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 282 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001588 (493 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4110
Search term: ampC

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter.
Lodge J, Busby S, Piddock L
FEMS Microbiol Lett 1993 Aug 1;111(2-3):315-20
PubMed ID: 8405939
A novel class C beta-lactamase (FOX-2) in Escherichia coli conferring resistance to cephamycins.
Bauernfeind A, Wagner S, Jungwirth R, Schneider I, Meyer D
Antimicrob Agents Chemother 1997 Sep;41(9):2041-6
PubMed ID: 9303413
Potentiation of beta-lactams against Pseudomonas aeruginosa strains by Ro 48-1256, a bridged monobactam inhibitor of AmpC beta-lactamases.
Livermore DM, Chen HY
J Antimicrob Chemother 1997 Sep;40(3):335-43
PubMed ID: 9338484