Pseudomonas aeruginosa PAO1, PA4191

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4191
Name
Replicon chromosome
Genomic location 4688889 - 4689893 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_252880.1
GI 15599386
Affymetrix PA4191_at
DNASU PaCD00007821
Entrez 880273
GenBank AAG07578.1
INSDC AAG07578.1
NCBI Locus Tag PA4191
protein_id(GenBank) gb|AAG07578.1|AE004835_7|gnl|PseudoCAP|PA4191
TIGR NTL03PA04191
UniParc UPI00000C5C64
UniProtKB Acc Q9HWJ0
UniProtKB ID Q9HWJ0_PSEAE
UniRef100 UniRef100_W0YWT2
UniRef50 UniRef50_Q3M330
UniRef90 UniRef90_W0YWT2

Product

Feature Type CDS
Coding Frame 1
Product Name
isopenicillin-N synthase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -6.65
Kyte-Doolittle Hydrophobicity Value -0.310
Molecular Weight (kDa) 37.4
Isoelectric Point (pI) 5.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6JYV METAL BINDING PROTEIN 04/28/19 Structure of an isopenicillin N synthase from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.651 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 170 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005153 (254 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4191

Human Homologs

References

Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.
Roach PL, Clifton IJ, Fülöp V, Harlos K, Barton GJ, Hajdu J, Andersson I, Schofield CJ, Baldwin JE
Nature 1995 Jun 22;375(6533):700-4
PubMed ID: 7791906
Structural characterization of an isopenicillin N synthase family oxygenase from Pseudomonas aeruginosa PAO1.
Zhang H, Che S, Wang R, Liu R, Zhang Q, Bartlam M
Biochem Biophys Res Commun 2019 Jul 5;514(4):1031-1036
PubMed ID: 31097228